MACS® GMP PepTivator® WT1 ( #170-076-123 )

MACS® GMP PepTivator® WT1

Overview

The MACS GMP PepTivator WT1 is a peptide pool that consists mainly of 15-mer peptides with eleven amino-acid overlaps. It has been developed for efficient in vitro stimulation and subsequent isolation of WT1-specific CD4⁺ and CD8⁺ T cells.

Details

Background information
The Wilms tumor antigen (WT1) is a zinc-finger transcription factor and is expressed during normal ontogenesis. Expression is absent or restricted to a very low level in adult healthy tissue. However, WT1 is expressed at high levels in various types of leukemia and solid tumors, including lung, breast, colon cancer and soft tissue sarcomas. The WT1 protein is highly immunogenic and has become an attractive target for anticancer immunotherapy.

Applications
Spontaneous humoral and cellular immune responses against WT1 have been described in patients with leukemia. Therefore WT1 has become an attractive target for tumor-directed immunotherapy.

The in vitro stimulation of WT1-specific CD4⁺ and CD8⁺ T cells with MACS GMP PepTivator WT1 causes them to produce the effector cytokine IFN-gamma. The presence of IFN-gamma on the cell surface then permits the enrichment of WT1-specific effector/memory T cells with the use of the CliniMACS Cytokine Capture System (IFN-gamma).

Adoptive transfer of WT1-specific T cells or vaccination with WT1-pulsed dendritic cells may be an effective tool for the treatment of WT-expressing malignancies.

Quality statement
MACS GMP Products are manufactured and tested under a certified ISO 9001 quality system and in compliance with relevant GMP guidelines. They are designed following the recommendations of USP <1043> on ancillary materials.

No animal-, human-, or virus-derived materials were used for manufacture of these products.

Disclaimer

MACS GMP Products are for research use and ex vivo cell culture processing only, and are not intended for human in vivo applications.