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| Overview |
| The CD24 MicroBead Kit has been developed and optimized for the depletion of CD24-expressing cells from cancer cell lines or single-cell suspensions created from solid tissues. It can also be used for positive selection of cancer stem cells from cancer cell lines or single-cell suspensions created from solid tissues. |
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Background information
The human CD24 antigen is also known as heat-stable antigen (HSA). CD24 has been identified to be a negative marker for breast cancer stem cells1 and a positive marker for ovarian2 or pancreatic cancer stem cells3. |
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| Figure 1 |
| CD24+ cells were depleted from the CML cell line K562 using the CD24 MicroBead Kit, an LD Column, and a QuadroMACS™ Separator. Cells were fluorescently labeled with CD24-PE and analyzed by flow cytometry using the MACSQuant® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence. |
| A: Before separation |
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| B: CD24- cell fraction |
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| Details |
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| References |
| 1. Al-Hajj et al. (2003) Proc Natl Acad Sci 100: 3983-3988 |
| 2. Gao et al. (2010) Oncogene 29: 2672-2680 |
| 3. Li et al. (2007) Cancer Res 67 (3): 1030-1037 |
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