CD44 MicroBeads
Description CD44 MicroBeads have been specifically designed and optimized for the isolation or depletion of CD44+ cells from solid tissue or cancer cell lines. CD44 is a marker for many types of cancer stem cells (CSC), including breast CSCs that possess higher tumorigenicity and metastatic potential1, colorectal2, pancreatic3, and prostate4,5 CSCs. In addition, expression was observed in several cancers as well as on carcinoma cell lines. Here, it plays a role in cancer cell migration and matrix adhesion in response to a cellular microenvironment, thus enhancing cellular aggregation and tumor cell growth6. CD44 is also expressed on mesodermal cells, such as hematopoietic, fibroblastic, and glial cells. CD44 MicroBeads are the first method for isolation of CD44+ cancer stem cells by direct magnetic labeling. Applications CD44 MicroBeads can be used for isolation or depletion of CD44+ cells from single-cell suspensions from solid tumors, including breast, pancreatic, colon, and prostate tissues or cancer cell lines. Columns For positive selection: LS, XS, or autoMACS® Columns; for depletion: LD, CS, D, or autoMACS Columns. Note: This product is not suitable for cell separation with an MS Column. Clone Isotype Further information Preparation of single-cell suspensions from human tumor tissues [PDF; 81,3 KB]		Figure 1 CD44+ cells were isolated from a mixture of U937 (CD44+) and 1881 (CD44–) cell lines. CD44+ cells were isolated using the CD44 MicroBeads, an LS Column, and a MidiMACS™ Separator. Cells were fluorescently stained with CD44-PE and analyzed by flow cytometry using the MACSQuant® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence. A: Before separation B: CD44– cells C: CD44+ cells

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