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| Anti-TRA-1-60 MicroBead Kit |
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| Overview |
The Anti-TRA-1-60 MicroBead Kit has been developed for the separation of human pluripotent stem cells based on the expression of the TRA-1-60 antigen.
The Anti-TRA-1-60 MicroBead Kit enables the:
- positive selection of undifferentiated TRA-1-60+ pluripotent stem cells, for example, human ES and iPS cells,
- isolation of undifferentiated cells from differentiated cell populations,
- efficient enrichment of iPS cells during or after reprogramming1.
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| Details |
Background information
The TRA-1-60 monoclonal antibody reacts with a pluripotent stem cell–specific antigen expressed on undifferentiated human embryonic stem (ES) cells, induced pluripotent (iPS) cells, embryonal carcinoma (EC) cells, and embryonic germ (EG) cells.2,3 The expression of TRA-1-60 on human ES cells is down-regulated upon differentiation. The TRA-1-60 antibody recognizes a neuraminidase-resistant carbohydrate epitope expressed on podocalyxin, a member of the CD34- related family of sialomucins. Podocalyxin is a transmembrane glycoprotein, which has been implicated in the development of aggressiveness in a variety of cancers including breast cancer and prostate cancer. |
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| Figure 1 |
| Pluripotent TRA-1-60+ iPS cells were isolated from cultures containing spontaneously differentiated iPS cells using the Anti-TRA-1-60 MicroBead Kit, an LS Column, and a MidiMACS™ Separator. Cells were fluorescently stained with Stemgent® Anti-TRA-1-60-PE Antibody and CD326-APC, and analyzed by flow cytometry using the MACSQuant® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence. |
| Before separation |
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| Enriched TRA-1-60+ pluripotent stem cells |
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| Favorites / Prices |
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| Details |
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| Products |
| Anti-TRA-1-60 MicroBead Kit, human |
- for 2×108 total cells
Download datasheet
130-095-816
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| References |
| 1. Dick, E. et al. (2011) Nat. Protocols 6 (6): 701-714. |
| 2. Andrews, P. W. et al. (1984) Hybridoma 3: 347–361. |
| 3. Chan, E. M. et al. (2009) Nat. Biotechnol. 27: 1033–1037. |
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