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Miltenyi Biotec Miltenyi Biotec MACS® Cell Separation Reagents | for human cells | Stem and progenitor cells | ESCs and iPS cells
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Cell separation reagents Cell separation reagents
  for human cells
  Stem and progenitor cells
 
 
 
 
 
 
  T cells
  NK cells
  B Cells
  Monocytes
  Dendritic cells
  Antigen-presenting cells
  Granulocytes and myeloid cells
  Leukocytes
  Erythroid cells
  Megakaryocytes and platelets
  Endothelial cells
  Epithelial cells
  Fibroblasts
  Neural cells
  Cytokine-producing cells
  Tumor cells
  Fc receptor blocking
  for non-human primate cells
  for mouse cells
  for rat cells
  for indirect magnetic labeling
  for apoptotic and dead cells
  for isolation of mitochondria
Manual cell separation Manual cell separation
Automated cell separation Automated cell separation
MACS® Technology MACS® Technology
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Pluripotent Stem Cell MicroBeads
Overview
Pluripotent Stem Cell MicroBeads were developed for the separation of human pluripotent stem cells based on the expression of the CD326 (EpCAM) antigen.

The Pluripotent Stem Cell MicroBeads can be used for the:

  • positive selection of undifferentiated CD326+ pluripotent stem cells, for example, human embryonic stem (ES) cells and induced pluripotent stem (iPS) cells
  • enrichment of iPS cells after reprogramming
Details
Background information
CD326 is expressed on undifferentiated human embryonic stem (ES) cells and induced pluripotent stem (iPS) cells and is localized to OCT4+ pluripotent cells. Its expression is down-regulated upon differentiation.¹,²
Columns
LS or autoMACS Columns
Further information
Stem Cell Research Brochure
[PDF; 3,8 MB]
Stem Cell Product List Sept 2011
[PDF; 709,8 KB]
 
Undifferentiated CD326+ iPS cells were isolated from cultures containing spontaneously differentiated iPS cells using Pluripotent Stem Cell MicroBeads, an LS Column, and a MidiMACS™ Separator. Cells were fluorescently stained with CD326 (EpCAM)-APC, Stemgent® Phycoerythrin (PE) Anti-Human TRA-1-60 Antibody, and analyzed by flow cytometry using the MACSQuant® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
Before separation
Enriched CD326+ pluripotent cells
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Details
Products
Pluripotent Stem Cell MicroBeads, human
- for 2×108 total cells
Download datasheet
130-095-804
Qty.:
 

Related products
hES Cell Cloning & Recovery Supplement (#130-095-690)
Stemolecule Thiazovivin (#130-095-568)
Human FGF-2
Human TGF-β1
Antibodies for human ES and iPS cell research
Anti-TRA-1-60 MicroBead Kit, human (#130-095-816)
Feeder Removal MicroBeads, mouse (#130-095-531)
Anti-SSEA-1 (CD15) MicroBeads, human and mouse (#130-094-530)
Stemedia™ NutriStem™ XF/FF Culture Medium
References
1. Ng et al. (2010) Stem Cells 28: 29–35.
2. Sundberg et al. (2009) Stem Cell Res. 2: 113–124.
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