Anti-SSEA-1 (CD15) MicroBeads
Description SSEA-1 is one of the hallmark mouse embryonic stem (ES) cell markers associated with the state of pluripotency and is known to be expressed on both undifferentiated ES and induced pluripotent stem (iPS) cells.1-4 In contrast, SSEA-1 is not expressed on undifferentiated human ES cells but on those having undergone spontaneous differentiation.5 Anti-SSEA-1 (CD15) MicroBeads are the first product to allow magnetic cell separation based on expression of SSEA-1 Pluripotent mouse ES or iPS cells can be enriched or depleted from mixed cultures within 1 hour Human ES or iPS cells undergoing spontaneous differentiation can also be depleted from mixed cultures within 1 hour Take a look through our stem cell pages to find out more about our range of solutions for ES and iPS cell research Applications Separation of mouse ES or iPS cells from co-cultured feeder cells Enrichment of reprogrammed mouse iPS cells Depletion of unwanted pluripotent cells from differentiation cultures derived from mouse ES or iPS cells Depletion of spontaneously differentiated cells from human ES or iPS cell cultures Columns LS Columns for both enrichment and depletion		Figure 1 Isolation of mouse ES cells using Anti-SSEA-1 (CD15) MicroBeads, an LS Column, and a MidiMACS™ Separator. Samples from a co-culture of mouse ES cells and embryonic fibroblasts expressing H-2Kk were taken either before or after MACS® Separation and stained with anti-H-2Kk-PE before analysis by flow cytometry. Expression of ectopic H-2Kk was performed solely as a means of identifying fibroblasts within mixed cultures and is not required for magnetic separation. Before separation Negative fraction Isolated mouse ES cells

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