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| Description |
The CD28 antibody is specific for the mouse CD28 costimulatory molecule, expressed on most thymocytes, CD4+ and CD8+ T cells and NK cells. Ligation of CD28 with CD80 (B7-1) and CD86 (B7-2) provides a costimulatory signal for T cell activation. Clone 37.51 has been shown to activate T cells in combination with CD3.
Activation and proliferation of T cells can be induced by interaction of the T cell receptor with peptide-MHC complexes. The T cell receives a signal transduced through the CD3 complex. Cytokines or other costimulatory signals from accessory cells are required in addition. Activated T cells can be used for any downstream processes, such as cytokine analysis or immunoprecipitation. Cells can also be transfected with high efficiency. |
| Applications |
| The antibody is suitable for in vitro T cell activation and expansion. T cells can be activated using the pure antibody in combination with CD3ε antibodies. CD28-Biotin can be used in combination with CD3ε-Biotin and Anti-Biotin MACSiBead™ Particles for activation and expansion of T cells. The antibody is free of azide and preservatives. Endotoxin level is <0.01 ng/µg protein as tested by LAL assay. |
| Clone | Isotype |
| 37.51 | Hamster IgG2 |
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