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| Anti-mPDCA-1 - functional grade |
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| Description |
Clone JF05-1C2.4.1 specifically recognizes the mouse plasmacytoid dendritic cell antigen-1 (mPDCA-1). mPDCA-1 is specifically expressed on mouse plasmacytoid dendritic cells (PDCs) — a subset of CD11c+ dendritic cells detected at low frequency in all lymphoid tissues, peripheral blood, and some non-lymphoid tissues.1
In mouse spleen, bone-marrow, and lymph nodes, mPDCA-1 is exclusively expressed on cells which are CD11clow, CD45R (B220)+, Ly-6Chigh, MHC Class IIlow, CD8alow/-, CD80low/-, CD86-, CD40-, CD11b-, CD90-, CD49b (DX5)-, CD3-, CD19-, i.e. on cells with the phenotype of mouse PDCs. Multi-color fluorescent staining of spleen cells clearly revealed that all CD11c+ CD45R (B220)+ Ly-6C+ PDCs are mPDCA-1+ and that mPDCA-1 expression is restricted to PDCs. In vivo injection of Anti-mPDCA-1 pure antibody efficiently depletes PDCs within 24 hours.2 |
| Applications |
| Anti-mPDCA-1 pure – functional grade is a special format of Anti-mPDCA-1 pure that was developed for the in vivo depletion of PDCs in order to study their functional role in experimental mouse models. The antibody has been used for PDC depletion in various mouse models of infection, for example, murine CMV2, cutaneous HSV3, and vaginal HSV-24 infection, where PDCs are shown to play a crucial role in antiviral protection. In addition, the functional role of PDCs after Listeria monocytogenes infection has been analyzed and revealed that the interaction between convential DCs and PDCs is essential for CpG-induced immune activation.5 |
| Clone | Isotype |
| JF05-1C2.4.1 | Rat IgG2b |
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| Figure 1 |
| Mouse PDCs were specifically depleted in BALB/c mice by i.p. injection of 500 µg Anti-mPDCA-1 pure – functional grade. The frequency of mPDCA-1+ PDCs was analyzed in spleen 24 h after injection of the antibody or diluent only (control mice) by staining with Anti-mPDCA-1. To exclude blocking of Anti-mPDCA-1 staining by the in vivo applied Anti-mPDCA-1 antibody, cells were additionally stained with Anti-Ly-6C and CD45R (B220). Note that the frequency of PDCs decreases in mice which received the Anti-mPDCA-1 antibody (A) to about one fifth compared to control mice (B), independent of whether it is analyzed based on Anti-mPDCA-1 staining or on Anti-Ly-6C and CD45R (B220) staining. Cell debris and dead cells were excluded from the analysis based on scatter signals and PI fluorescence. |
| A) Anti-mPDCA-1 antibody |
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| B) Control |
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| MACS References |
| 1. Fischer et al. (2004) MACS&more 8-1: 13–15.[7427] |
| 2. Krug et al. (2004) Immunity 21: 107–119.[4201] |
| 3. Yoneyama et al. (2005) J. Ex. Med. 202(3): 425–435.[7569] |
| 4. Shen et al. (2006) J. Clin. Invest. 116: 2237–2243.[9257] |
| 5. Kuwajima et al. (2006) Nat. Immunol. 7: 740–746.[8806] |
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