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| Overview |
The antibody is suitable for in vitro T cell activation and expansion. T cells can be activated using the pure antibody in combination with CD3 antibodies. CD28-Biotin can be used in combination with CD2-Biotin, CD3-Biotin, and Anti-Biotin MACSiBead™ Particles for activation and expansion of T cells.
The antibody is free of azide and preservatives. Endotoxin level is <0.01 ng/µg of protein as tested by LAL assay. |
| Details |
Background information
The CD28 antibody recognizes the human CD28 antigen, which is highly expressed on CD3+ thymocytes, most peripheral thymocytes and plasma cells. Binding of CD28 to its ligands CD80 or CD86 costimulates T cell effector function and T cell–dependent antibody production in vitro and in vivo.
In the presence of antibodies directed against CD2 and CD3, CD28 antibodies stimulate T cell proliferation and cytokine production.
Activation and proliferation of T cells can be induced by interaction of the T cell receptor with peptide-MHC complexes expressed on the surface of APCs. The T cell receives a signal transduced through the CD3 complex. Cytokines or other costimulatory signals are required in addition. Activated T cells can be used for any downstream processes, such as cytokine analysis or immunoprecipitation. Cells can also be transfected with high efficiency. |
| Cross-reactivity |
The antibody was tested to cross-react with
- Rhesus monkey (Macaca mulatta)
- Cynomolgus monkey (Macaca fascicularis)
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| Clone | Isotype |
| 15E8 | Mouse IgG1 |
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