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| Overview |
The antibody is suitable for in vitro human T cell activation and expansion. T cells can be activated using the pure antibody alone or in combination with CD28 antibodies. CD3-Biotin can be used in combination with CD2-Biotin, CD28-Biotin, and Anti-Biotin MACSiBead™ Particles for activation and expansion of T cells.
The antibody is free of azide and preservatives. Endotoxin level is <0.01 ng/µg of protein as tested by LAL assay. |
| Details |
Background information
The CD3 antibody recognizes the human CD3 antigen which is present on mature human T cells, thymocytes, and NKT cells. The epitope recognized by the antibody is located on the ε-chain of the CD3 complex. Clone OKT3 has been shown to activate T cell function.
Activation and proliferation of T cells can be induced by interaction of the T cell receptor with peptide-MHC complexes expressed on the surface of APCs. The T cell receives a signal transduced through the CD3 complex. Cytokines or other costimulatory signals are required in addition. Activated T cells can be used for any downstream processes, such as cytokine analysis or immunoprecipitation. Cells can also be transfected with high efficiency. |
| Clone | Isotype |
| OKT3 | Mouse IgG2a |
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