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| Overview |
IL-6 stands for interleukin 6. Mouse IL-6 is a recombinant protein optimized for use in cell culture, differentiation studies, and functional assays.
Human IL-6 can be used for a variety of applications, including:
- Induction of colony formation from hematopoietic progenitor cells in semi-solid medium.
- Replacement of feeder cells in the preparation of murine hybridomas.
- In vitro differentiation of TH17 cells.
- Cultivation of bone marrow cells for retroviral transduction.
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| Details |
Background information
IL-6, originally identified as a B cell differentiation factor, is a multifunctional cytokine which regulates immune responses, hematopoiesis, acute phase responses, and inflammatory reactions. It induces, for instance, the terminal maturation of activated B cells into antibody-secreting plasma cells and acts in synergy with IL-3 to support the proliferation of hematopoietic stem cells. IL-6 is produced by many cell types, such as monocytes, macrophages, fibroblasts, endothelial cells, and T cells.
Quality description
Research-grade cytokines are suitable for a wide variety of cell culture applications. They are sterile-filtered prior to lyophilization. Generally, endotoxin levels are <0.1 ng/µg (<1 EU/µg), and purities are >95%. The biological activity is tested in appropriate bioassays.
Premium-grade cytokines offer the convenience of high and well-defined biological activities and allow exact unit dosing for demanding applications. The biological activity is determined after lyophilization and reconstitution, and normalized to WHO/NIBSC standards whenever available. In general, endotoxin levels are <0.01 ng/µg (<0.1 EU/µg), and purities are >97%. Lot-specific certificates of analysis are available on request (macstec@miltenyibiotec.de). |
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| Figure 1 |
| Mouse IL-6, activity assay. The biological activity was determined by proliferation assay using mouse 7TD1 cells. Activity of Mouse IL-6, premium grade (red line) was compared to another commercially available product (black line). |
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| Figure 2 |
| SDS-PAGE of Mouse IL-6, premium grade under reduced (R) and non-reduced (NR) conditions. |
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