Mouse IFN-α ( #130-093-131 #130-093-130 )

Mouse IFN-α

Overview

IFN-α stands for interferon alpha. Mouse IFN-α is a recombinant protein optimized for use in cell culture, differentiation studies, and functional assays.

Mouse IFN-α can be used for studies of in vitro and in vivo effects of IFN-α in mouse models, e.g., for the in vitro investigation of antitumor effects (antiproliferative capacities) of IFN-α on tumor cell lines or for stimulation of IFN-α-dependent cell lines.
In vivo, recombinant mouse IFN-α can be utilized in studies using transgenic mice harboring the Cre-recombinase transgene driven by the interferon-inducible Mx promoter (Mx-Cre mice)³.

Details

Background information
Type I interferons (IFNs), including IFN-α, are a family of cytokines that exert multiple functions in the immune system^1,2. The most prominent effect of IFN-α is its antiviral activity. Upon viral infections, host cells release IFN-α which can act in an autocrine or paracrine manner to activate intracellular antiviral defense mechanisms and restrict viral replication. Furthermore, IFN-α affects the generation and function of various dendritic cell populations. Immunomodulatory activity and antitumor effects have been described both in vivo and in vitro.
The recombinant Mouse IFN-α was expressed in HEK293 cells and has been purified and analyzed for its activity (10⁷ U/mL).

Quality description
Research-grade cytokines are suitable for a wide variety of cell culture applications. They are sterile-filtered prior to lyophilization. Generally, endotoxin levels are <0.1 ng/µg (<1 EU/µg), and purities are >95%. The biological activity is tested in appropriate bioassays.

Figure 1

Mouse IFN-α activity assay. The biological activity of Mouse IFN-α was determined by inhibition assay using a T lymphoma cell line (BW cells).