|
|
 |
|
| Overview |
IL-23 stands for interleukin 23. Mouse IL-23 is a recombinant protein optimized for use in cell culture, differentiation studies, and functional assays.
Mouse IL-23 can be used for a variety of applications, including:
- In vitro differentiation of CD4+ T cells towards TH17 cells and proliferation of TH17 cells.
- Exploration of the role of IL-23 in autoimmune diseases or inflammatory bowel diseases.
- Investigation of IL-23-mediated molecular signaling pathways.
|
| Details |
Background information
IL-23 is a covalently linked heterodimeric cytokine, which is closely related to IL-12. Macrophages and dendritic cells are the major sources of both IL-12 and IL-23, which act on receptors primarily expressed by T cells, NK cells, and NKT cells. IL-23 consists of the p40 subunit shared with IL-12 and the 19 kDa protein p19 that is unique to IL-23. Its effects are mediated by a receptor composed of IL-12 receptor subunit β1 and an IL-23 specific subunit. IL-12 and IL-23 show different functions although both contain the p40 subunit. While IL-12 drives the development of TH1 cells, IL-23 mediates the full differentiation and maintenance of TH17 cells. IL-23 stimulates CD4+ T cells and promotes the production of pro-inflammatory cytokines. As a key cytokine in the survival and proliferation of TH17 cells, IL-23 has central roles in autoimmune diseases and promotes chronic inflammation. It has been shown that single chain fusion proteins of naturally heterodimeric cytokines such as IL-12 or IL-23 are bioactive in vitro and in vivo.
Quality description
Research-grade cytokines are suitable for a wide variety of cell culture applications. They are sterile-filtered prior to lyophilization. Generally, endotoxin levels are <0.1 ng/µg (<1 EU/µg), and purities are >95%. The biological activity is tested in appropriate bioassays. |
| |
| Figure 1 |
| Mouse IL-23 activity assay. The biological activity of Mouse IL-23 was determined by assessment of IL-17 secretion by mouse splenocytes activated with 10 ng/mL PMA. |
 |
|
| Figure 2 |
| SDS-PAGE of Mouse IL-23 under reducing conditions (R). Units p40 and p19 are fused by a flexible linker region resulting in a single-chain polypeptide. The theoretical molecular mass is 58 kDa. Due to glycosylation the protein has an apparent mass of 70 kDa. |
 |
|
|
|
|
| Favorites / Prices |
Request prices or add products to your favorite list.
|
| Details |
|
|
|
© 1999 - 2012 Miltenyi Biotec ¦ Terms & conditions ¦ Legal notes
|
