|
|
 |
|
| Overview |
IL-12 stands for interleukin 12. Mouse IL-12 is a recombinant protein optimized for use in cell culture, differentiation studies, and functional assays.
Mouse IL-12 can be used for a variety of applications, including:
- In vitro activation and proliferation of NK and T cells.
- In vitro differentiation of naive CD4+ T cells towards TH1 cells.
- In vitro stimulation of cytotoxic activity of NK cells and T cells.
|
| Details |
Background information
IL-12 is a heterodimeric proinflammatory cytokine and a modulator of cell-mediated immunity, which is mainly produced by macrophages, dendritic cells, and B cells. IL-12 stimulates the production and secretion of several cytokines, in particular IFN-γ, by NK cells and T cells, induces proliferation and enhances the cytotoxic activity within these cell populations. Another important activity of IL-12, acting together with IFN-γ and IL-2, is to drive T helper cell responses toward the TH1 rather than the TH2 phenotype. Moreover, IL-12 is also important in resistance to viral disease and has significant antitumor activity. It has been shown that single chain fusion proteins of naturally occurring heterodimeric cytokines such as IL-12 or IL-23 are bioactive in vitro and in vivo.
Quality description
Research-grade cytokines are suitable for a wide variety of cell culture applications. They are sterile-filtered prior to lyophilization. Generally, endotoxin levels are <0.1 ng/µg (<1 EU/µg), and purities are >95%. The biological activity is tested in appropriate bioassays. |
| |
| Figure 1 |
| Mouse IL-12 activity assay. The biological activity of mouse IL-12 is determined by induction of IFN-γ secretion by splenic T cells. |
 |
|
| Figure 2 |
| SDS-PAGE of Mouse IL-12 under reduced (R) and non-reduced (NR) conditions. |
 |
|
|
|
|
| Favorites / Prices |
Request prices or add products to your favorite list.
|
| Details |
|
|
|
© 1999 - 2012 Miltenyi Biotec ¦ Terms & conditions ¦ Legal notes
|
