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| Overview |
LIF stands for leukemia inhibitory factor. Mouse LIF is a recombinant protein optimized for use in cell culture, differentiation studies, and functional assays.
Mouse LIF is essential for the maintenance of self-renewal and pluripotency in conventional mouse ESC cultures.
A minimal concentration of 10 ng/mL or 20 units/mL are required to support mouse ESC in an undifferentiated state. |
| Details |
Background information
LIF is a pleiotropic cytokine, which is critically involved in embryonic development and blastocyst implantation. LIF belongs to the interleukin 6 family and functions through the gp130 activation of STAT3. In mice LIF is a key factor that prevents embryonic stem cells (ESC) to differentiate. Additionally, LIF affects hematopoiesis, neural development, bone and energy metabolism, and inflammation.
Quality description
Research-grade cytokines are suitable for a wide variety of cell culture applications. They are sterile-filtered prior to lyophilization. Generally, endotoxin levels are <0.1 ng/µg (<1 EU/µg), and purities are >95%. The biological activity is tested in appropriate bioassays.
Premium-grade cytokines offer the convenience of high and well-defined biological activities and allow exact unit dosing for demanding applications. The biological activity is determined after lyophilization and reconstitution, and normalized to WHO/NIBSC standards whenever available. In general, endotoxin levels are <0.01 ng/µg (<0.1 EU/µg), and purities are >97%. Lot-specific certificates of analysis are available on request (macstec@miltenyibiotec.de). |
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| Figure 1 |
| Mouse LIF activity assay. The biological activity of Mouse LIF was determined by inhibition assay using mouse M1 cells. Activity of Mouse LIF, premium grade (red line) was compared to another commercially available product (black line) with fully equivalent results. |
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| Figure 2 |
| SDS-PAGE of Mouse LIF, premium grade under reduced (R) and non-reduced (NR) conditions. |
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