Human IL-12 ( #130-096-704 #130-096-705 #130-096-798 )

Human IL-12

Overview

IL-12 stands for interleukin 12. Human IL-12 is a recombinant protein optimized for use in cell culture, differentiation studies, and functional assays.

Human IL-12 can be used for a variety of applications, including:

In vitro activation and proliferation of NK and T cells.
In vitro differentiation of naive CD4⁺ T cells towards TH1 cells.
In vitro stimulation of cytotoxic activity of NK cells and T cells.

Details

Background information
IL-12 is a heterodimeric proinflammatory cytokine and a modulator of cell-mediated immunity, which is mainly produced by macrophages, dendritic cells, and B cells. IL-12 stimulates the production and secretion of several cytokines, in particular IFN-γ, by NK cells and T cells, induces proliferation and enhances the cytotoxic activity within these cell populations. Another important activity of IL-12, acting together with IFN-γ and IL-2, is to drive T helper cell responses toward the T_H1 rather than the T_H2 phenotype. Moreover, IL-12 is also important in resistance to viral disease and has significant antitumor activity. It has been shown that single chain fusion proteins of naturally occurring heterodimeric cytokines such as IL-12 or IL-23 are bioactive in vitro and in vivo.

Quality description
Premium-grade cytokines offer the convenience of high and well-defined biological activities and allow exact unit dosing for demanding applications. The biological activity is determined after lyophilization and reconstitution, and normalized to WHO/NIBSC standards whenever available. In general, endotoxin levels are <0.01 ng/µg (<0.1 EU/µg), and purities are >97%. Lot-specific certificates of analysis are available on request (macstec@miltenyibiotec.de).

Figure 1

Human IL-12 activity assay. The biological activity of Human IL-12 is determined by induction of IFN-γ secretion by PHA-activated T cells.

Figure 2

SDS-PAGE of Human IL-12, premium grade under reduced (R) and non-reduced (NR) conditions.