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| Description |
Interleukin 6 (IL-6), originally identified as a B cell differentiation factor, is a multifunctional cytokine, which regulates immune responses, hematopoiesis, acute phase responses, and inflammatory reactions. It induces, for instance, the terminal maturation of activated B cells into antibody-secreting plasma cells and acts in synergy with IL-3 to support the proliferation of hematopoietic stem cells. IL-6 is produced by many cell types, such as monocytes, fibroblasts, endothelial cells, T cells, etc. Disturbed IL-6 production has been associated with pathological processes including inflammatory autoimmune diseases, such as rheumatoid arthritis, and cancer.
Applications
Human IL-6 can be used for a variety of applications, including:
- induction of colony formation from hematopoietic progenitor cells in semi-solid medium,
- replacement of feeder cells in the preparation of murine and human hybridomas,
- in vitro differentiation of TH17 cells.
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| Quality description |
Research grade
Research grade is the standard quality for all our products. They are sterile-filtered and have passed an accurate quality control. Generally, endotoxin levels are below 0.1 ng/µg (< 1 EU/µg), and purities are higher than 95%. The biological activity has usually been tested employing appropriate bioassays, and a minimum activity is assured. Otherwise the ED50 value is given.
Premium grade
For demanding applications, selected human cytokines and growth factors are available in premium grade quality having a particular high and well-defined biological activity. They have passed stringent quality control criteria, and the purity is higher than 97%. The biological activity has been determined after lyophilization in direct comparison with the respective WHO standards. Certificates of analysis stating lot-specific biological activities are available on request. Furthermore, premium grade cytokines display the major characteristics of our line of GMP grade cytokines. Premium grade products are widely used, e.g, for the generation of dendritic cells and the cultivation of stem cells. |
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| Figure 1 |
| Human IL-6 activity assay. The biological activity of Human IL-6, premium grade was determined by proliferation assay using B9 hybridoma cells. |
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