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| Overview |
IL-4 stands for Interleukin 4. Human IL-4 is a recombinant protein optimized for use in cell culture, differentiation studies, and functional assays.
Human IL-4 can be used for a variety of applications including:
- In vitro generation of Mo-DCs (e.g. together with GM-CSF)1.
- In vitro differentiation of naive CD4+ T cells towards TH2 cells.
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| Details |
Background information
IL-4 is a pleiotropic cytokine, which plays a central role in humoral and adaptive immune responses. IL-4 is predominantly secreted by activated CD4+ memory and effector TH2 cells, basophils, and mast cells. It promotes the proliferation and differentiation of B cells, as well as immunoglobulin isotype switching, and MHC class II antigen and low-affinity IgE receptor expression. Furthermore, IL-4 induces the differentiation of naive CD4+ T cells into helper TH2 cells, while suppressing TH1 development, and promotes chemotaxis of mast cells and basophils. Excessive IL-4 production and mechanisms involving TH2 types have been associated with immunological disorders, such as IgE-mediated allergy.
Quality description
Research-grade cytokines are suitable for a wide variety of cell culture applications. They are sterile-filtered prior to lyophilization. Generally, endotoxin levels are <0.1 ng/µg (<1 EU/µg), and purities are >95%. The biological activity is tested in appropriate bioassays.
Premium-grade cytokines offer the convenience of high and well-defined biological activities and allow exact unit dosing for demanding applications. The biological activity is determined after lyophilization and reconstitution, and normalized to WHO/NIBSC standards whenever available. In general, endotoxin levels are <0.01 ng/µg (<0.1 EU/µg), and purities are >97%. Lot-specific certificates of analysis are available on request (macstec@miltenyibiotec.de).
Cell culture grade cytokines and growth factors are lyophilized with DMEM/F12 and fetal bovine serum or human serum albumin. These products are well-suited for certain applications where the direct reconstitution with water is favorable, e.g., applications with small volumes and tests in 96-well plates. |
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| Figure 1 |
| Human IL-4 activity assay. The biological activity of Human IL-4, premium grade was determined by proliferation assay using TF-1 cells. |
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| Details |
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| References |
| 1. Kandler et al. (2006) Int. Immunol. 18: 1729–1736. |
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