Human IL-3 ( #130-093-908 #130-093-909 #130-095-071 #130-095-070 #130-095-069 #130-095-068 )

Human IL-3

Description

Interleukin 3 (IL-3) is a hematopoietic growth factor which is produced mainly by activated T cells, but is also secreted by other cell types, including mast cells, eosinophils, and keratinocytes. The broad spectrum of biological activities of IL-3 includes the stimulation of the proliferation and differentiation of immature pluripotent hematopoietic stem cells and various lineage-committed progenitor cells, leading to the production of most of the major blood cell types. In addition, IL-3 also affects the functional activity of mature mast cells, basophils, eosinophils, and macrophages.

Applications
Human IL-3 can be used for a variety of applications, including:

induction of colony formation from hematopoietic progenitor cells in semi-solid medium in vitro,
in vitro differentiation studies,
cultivation of plasmacytoid dendritic cells¹,
investigation of mast cell or basophil function,
investigation of IL-3–mediated signaling pathways.

Quality description

Research grade
Research grade is the standard quality for all our products. They are sterile-filtered and have passed an accurate quality control. Generally, endotoxin levels are below 0.1 ng/µg (< 1 EU/µg), and purities are higher than 95%. The biological activity has usually been tested employing appropriate bioassays, and a minimum activity is assured. Otherwise the ED₅₀ value is given.

Premium grade
For demanding applications, selected human cytokines and growth factors are available in premium grade quality having a particular high and well-defined biological activity. They have passed stringent quality control criteria, and the purity is higher than 97%. The biological activity has been determined after lyophilization in direct comparison with the respective WHO standards. Certificates of analysis stating lot-specific biological activities are available on request. Furthermore, premium grade cytokines display the major characteristics of our line of GMP grade cytokines. Premium grade products are widely used, e.g, for the generation of dendritic cells and the cultivation of stem cells.

Figure 1

Human IL-3 activity assay. The biological activity of Human IL-3, premium grade was determined by proliferation assay using TF-1 cells.

Figure 2

SDS-PAGE of Human IL-3, premium grade from Miltenyi Biotec (MB) in comparison to recombinant human IL-3 from a different supplier (S), both under reduced and non-reduced conditions.