Human IL-3 ( #130-093-908 #130-093-909 #130-095-071 #130-095-070 #130-095-069 #130-095-068 )

Human IL-3

Overview

IL-3 stands for interleukin 3. Human IL-3 is a recombinant protein optimized for use in cell culture, differentiation studies, and functional assays.

Human IL-3 can be used for a variety of applications, including:

Induction of colony formation from hematopoietic progenitor cells in semi-solid medium in vitro.
In vitro differentiation studies.
Cultivation of plasmacytoid dendritic cells^1_.
In vitro expansion of hematopoietic stem cells^2_.
Investigation of mast cell or basophil function.
Investigation of IL-3–mediated signaling pathways.

Details

Background information
IL-3 is a hematopoietic growth factor, which is produced mainly by activated T cells, but is also secreted by other cell types, including mast cells, eosinophils, and keratinocytes. The broad spectrum of biological activities of IL-3 includes the stimulation of the proliferation and differentiation of immature pluripotent hematopoietic stem cells and various lineage-committed progenitor cells, leading to the production of most of the major blood cell types. In addition, IL-3 also affects the functional activity of mature mast cells, basophils, eosinophils, and macrophages.

Quality description
Research-grade cytokines are suitable for a wide variety of cell culture applications. They are sterile-filtered prior to lyophilization. Generally, endotoxin levels are <0.1 ng/µg (<1 EU/µg), and purities are >95%. The biological activity is tested in appropriate bioassays.
Premium-grade cytokines offer the convenience of high and well-defined biological activities and allow exact unit dosing for demanding applications. The biological activity is determined after lyophilization and reconstitution, and normalized to WHO/NIBSC standards whenever available. In general, endotoxin levels are <0.01 ng/µg (<0.1 EU/µg), and purities are >97%. Lot-specific certificates of analysis are available on request (macstec@miltenyibiotec.de).

Figure 1

Human IL-3 activity assay. The biological activity of Human IL-3, premium grade was determined by proliferation assay using TF-1 cells.

Figure 2

SDS-PAGE of Human IL-3, premium grade from Miltenyi Biotec (MB) in comparison to recombinant human IL-3 from a different supplier (S), both under reducing and non-reducing conditions.