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| Overview |
IFN-γ stands for interferon γ. Human IFN-γ is a recombinant protein optimized for use in cell culture, differentiation studies, and functional assays.
Human IFN-γ1b can be used for a variety of applications, including:
- Stimulation of monocytes, macrophages, and microglia.
- In vitro maturation of Mo-DCs.
- Apoptosis and cell growth inhibition assays.
- Adhesion and migration assays.
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| Details |
Background information
IFN-γ, the only type II interferon, possesses potent anti-viral activity and immunoregulatory properties. It is structurally unrelated to the type I interferons and binds to a different receptor expressed by most immune cells. IFN-γ is produced predominantly by NK cells, and T cells. IFN-γ enhances NK cell and macrophage activity and regulates B cell functions, like immunglobulin production and class switching. In addition, IFN-γ orchestrates leukocyte attraction and directs growth, maturation, and differentiation of many cell types. The activity of human IFN-γ is species-specific.
Quality description
Research-grade cytokines are suitable for a wide variety of cell culture applications. They are sterile-filtered prior to lyophilization. Generally, endotoxin levels are <0.1 ng/µg (<1 EU/µg), and purities are >95%. The biological activity is tested in appropriate bioassays.
Premium-grade cytokines offer the convenience of high and well-defined biological activities and allow exact unit dosing for demanding applications. The biological activity is determined after lyophilization and reconstitution, and normalized to WHO/NIBSC standards whenever available. In general, endotoxin levels are <0.01 ng/µg (<0.1 EU/µg), and purities are >97%. Lot-specific certificates of analysis are available on request (macstec@miltenyibiotec.de). |
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| Figure 1 |
| Human IFN-γ1b, activity assay. The biological activity of Human IFN-γ1b is determined by inhibition assay using HT-29 cells. Activity of Human IFN-γ, premium grade (red line) was compared to another commercially available product (black line). |
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| Figure 2 |
| SDS-PAGE of Human IFN-γ1b, premium grade under reduced (R) and non-reduced (NR) conditions. |
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