|
|
 |
|
| Description |
Human granulocyte-macrophage colony–stimulating factor (GM-CSF) is a hematopoietic growth factor, which is essential for proliferation and development of granulocyte and monocyte/macrophage progenitors. It also functions as a growth factor for erythroid and megakaryocytic precursor cells in conjunction with erythropoietin. GM-CSF is secreted by various cell types including T cells, macrophages, endothelial cells, and fibroblasts in response to inflammatory stimuli and cytokines. In addition, GM-CSF is a potent chemoattractant for neutrophils and eosinophils and enhances the effector functions of neutrophils and macrophages.
Applications
Human GM-CSF can be used for a variety of applications including:
- cultivation of hematopoietic progenitor cells from human bone marrow in semi-solid medium,
- in vitro generation of Mo-DCs (e.g. together with IL-4)1,
- in vitro differentiation of CD34+ cells towards eosinophils,
- migration assays for eosinophils.
|
| Quality description |
Research grade
Research grade is the standard quality for all our products. They are sterile-filtered and have passed an accurate quality control. Generally, endotoxin levels are below 0.1 ng/µg (< 1 EU/µg), and purities are higher than 95%. The biological activity has usually been tested employing appropriate bioassays, and a minimum activity is assured. Otherwise the ED50 value is given.
Premium grade
For demanding applications, selected human cytokines and growth factors are available in premium grade quality having a particular high and well-defined biological activity. They have passed stringent quality control criteria, and the purity is higher than 97%. The biological activity has been determined after lyophilization in direct comparison with the respective WHO standards. Certificates of analysis stating lot-specific biological activities are available on request. Furthermore, premium grade cytokines display the major characteristics of our line of GMP grade cytokines. Premium grade products are widely used, e.g, for the generation of dendritic cells and the cultivation of stem cells. |
| |
| Figure 1 |
| Human GM-CSF activity assay. The biological activity of Human GM-CSF, premium grade was determined by proliferation assay using TF-1 cells. |
 |
|
|
|
|
| My favorites |
| Your current favorite list is empty. |
|
| References |
| 1. Kandler et al. (2006) Int. Immunol. 18: 1729–1736. |
|
|
|
© 1999 - 2010 Miltenyi Biotec ¦ Terms & conditions ¦ Legal notes
|
