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| Overview |
Flt3-Ligand stands for Fms-related tyrosine kinase 3 ligand. Human Flt3-Ligand is a recombinant protein optimized for use in cell culture, differentiation studies, and functional assays.
Human Flt3-Ligand can be used for a variety of applications, including:
- In vitro expansion of CD34+ hematopoietic progenitor cells.
- Differentiation of ES-derived cells towards the hematopoietic lineage.
- In vitro generation of Langerhans cells, dendritic cells, or eosinophils from CD34+ cells.
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| Details |
Background information
Flt3-Ligand is a growth factor that regulates early hematopoiesis. Flt3-Ligand belongs to a small family of α-helical cytokines and promotes in synergy with other growth factors like G-CSF, GM-CSF, SCF, and IL-3 the proliferation and differentiation of primitive hematopoietic stem cells. Early B cell lineage differentiation as well as expansion of monocytes and immature dendritic cells is stimulated. Flt3-Ligand is expressed by T lymphocytes and bone marrow stromal fibroblasts as a membrane-bound and a soluble isoform. Both isoforms signal through the tyrosine kinase receptor Flt3/Flk-2, which is restricted to cells of hematopoietic origin. Human Flt3-Ligand is also active on mouse cells.
Quality description
Research-grade cytokines are suitable for a wide variety of cell culture applications. They are sterile-filtered prior to lyophilization. Generally, endotoxin levels are <0.1 ng/µg (<1 EU/µg), and purities are >95%. The biological activity is tested in appropriate bioassays.
Premium-grade cytokines offer the convenience of high and well-defined biological activities and allow exact unit dosing for demanding applications. The biological activity is determined after lyophilization and reconstitution, and normalized to WHO/NIBSC standards whenever available. In general, endotoxin levels are <0.01 ng/µg (<0.1 EU/µg), and purities are >97%. Lot-specific certificates of analysis are available on request (macstec@miltenyibiotec.de). |
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| Figure 1 |
| Human Flt3-Ligand activity assay. The biological activity of Human Flt3-Ligand is determined by proliferation assay using OCI-AML5 cells. |
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| Figure 2 |
| SDS-PAGE of Human Flt3-Ligand, premium grade under reduced (R) and non-reduced (NR) conditions. |
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