|
|
 |
|
| Overview |
IL-1β stands for interleukin-1 beta. Human IL-1β is a recombinant protein optimized for use in cell culture, differentiation studies, and functional assays.
Human IL-1β can be used for a variety of applications including:
- Induction of Mo-DC maturation.
- Chemotaxis assays.
- Investigation of IL-1 receptor signaling.
|
| Details |
Background information
IL-1β is a proinflammatory cytokine that is secreted mainly by monocytes and macrophages. IL-1β secretion has also been reported for a variety of other cells, including B cells, NK cells, dendritic cells, astrocytes, and microglial cells. It mediates inflammatory responses in B cells, T cells, and NK cells by inducing the production of cytokines, such as IL-2, IL-3, IL-6, as well as interferons. Upon exposure to IL-1β, endothelial cells and smooth muscle cells synthesize prostaglandins and other derivatives of arachidonic acid.
In addition, IL-1β is found in synovial fluid of arthritis patients, causing degranulation of basophils and eosinophils as well as activation of osteoclasts. IL-1β is mitogenic for mesangial cells, glial cells, and keratinocytes.
Quality description
Research-grade cytokines are suitable for a wide variety of cell culture applications. They are sterile-filtered prior to lyophilization. Generally, endotoxin levels are <0.1 ng/µg (<1 EU/µg), and purities are >95%. The biological activity is tested in appropriate bioassays.
Premium-grade cytokines offer the convenience of high and well-defined biological activities and allow exact unit dosing for demanding applications. The biological activity is determined after lyophilization and reconstitution, and normalized to WHO/NIBSC standards whenever available. In general, endotoxin levels are <0.01 ng/µg (<0.1 EU/µg), and purities are >97%. Lot-specific certificates of analysis are available on request (macstec@miltenyibiotec.de). |
| |
| Figure 1 |
| Human IL-1β activity assay. The biological activity of Human IL-1β, premium grade is determined by proliferation assay using D10.G4.1 cells. |
 |
|
| Figure 2 |
| SDS-PAGE of Human IL-1β, premium grade under reduced (R) and non-reduced (NR) conditions. |
 |
|
|
|
|
| Favorites / Prices |
Request prices or add products to your favorite list.
|
| Details |
|
|
|
© 1999 - 2012 Miltenyi Biotec ¦ Terms & conditions ¦ Legal notes
|
