| Description |
Endotoxin Removal Beads represent the ideal tool for fast and easy removal of endotoxin and DNA from protein preparations.
Preparations of recombinant proteins are often contaminated with endotoxins (lipopolysaccharides, LPS) and DNA from host bacteria. Bacterial endotoxins and DNA show strong biological effects in many mammals as well as in cell cultures. If purified recombinant proteins are used for in vivo or in vitro assays, efficient removal of endotoxins and bacterial DNA is required to avoid non-specific detrimental effects.
The covalently bound polycationic moiety of the Endotoxin Removal Beads shows strong binding to both endotoxins and DNA. |
| Applications |
Endotoxin Removal Beads have been developed for the removal of endotoxin and DNA from purified protein preparations.
Endotoxin or DNA can be removed by 98% at initial concentrations ranging from 2000 EU/mL to 10,000 EU/mL or 2000 to 10,000 ng of DNA per mL, respectively. |
| Required equipment |
Endotoxin removal is performed using the MACSiMAG™ Separator.
Note: Endotoxin Removal Beads are not suitable for use with MACS® Columns and MiniMACS™, MidiMACS™, VarioMACS™, SuperMACS™, autoMACS™, μMACS™, or thermoMACS™ Separators. |
| |
| Figure 1 |
| Removal of endotoxins from various sources out of a 1.0 mg/mL BSA solution in 0.1 mol/L sodium-phosphate buffer. The Endotoxin Removal Bead–concentration is 15 mg/mL. BSA recovery was above 84% at any rate. |
 |
|
| Figure 2 |
| Correlation of endotoxin removal and protein recovery. The Endotoxin Removal Bead concentration is 15 mg per mL of 0.1 M Na2HPO4 buffer. The incubation time is 10 minutes. |
 |
|
|
