Neural Tissue Dissociation Kits
Description The Neural Tissue Dissociation Kits, based on papain and trypsin, have been developed for the gentle and efficient generation of single-cell suspensions from neural tissues, and can be usede in combination with the gentleMACS™ Dissociator . In less than an hour, the gentle two-step enzymatic dissociation procedure efficiently yields high numbers of viable cells (figure 1). Neural tissues from embryonic, postnatal, and adult mouse or rat tissue sections or whole brain preparations can be processed. The enzyme mixes provided in the papain-based Neural Tissue Dissociation Kit (P) do not contain trypsin, whereas the enzyme mixes of the trypsin-based Neural Tissue Dissociation Kit (T) do not contain papain. For optional further repetitions of the incubation with Enzyme Mix 2 as described within the DataSheet, a supplement for the Neural Tissue Dissociation Kit Enzyme Mix 2 is available. The dissociated cells are immediately ready for further in vitro or in vivo applications, including MACS® Cell Separations. Applications Dissociation of neural tissues to single-cell suspensions, e.g., for subsequent cell separations using MACS Technology or further in vitro and in vivo applications. The kit based on papain is best suited for use with Anti-Prominin-1 MicroBeads and Anti-A2B5 MicroBeads, whereas the kit based on trypsin is best suited for use with Anti-PSA-NCAM MicroBeads. CD11b (Microglia) MicroBeads are suitable for use with both kits. The papain-based kit is recommended for use with all other epitopes. Please see figure 2 and 3 as well as the application note in the download section for further information with regards to kit selection in relation to subsequent neural cell separation using MACS® Technology. Further information Neural Tissue Dissociation Kit Flyer [PDF; 147,7 KB] Neuroscience research flyer [PDF; 623,8 KB] Neural Tissue Dissociation Kit application note [PDF; 66,8 KB] Preparation of single-cell suspensions from mouse neural tissue [PDF; 114,6 KB] Scientific poster – Neural tissue dissociation [PDF; 4 MB] gentleMACS Dissociator flyer [PDF; 83,3 KB] Schwann cell isolation [PDF; 185 KB]		Figure 1 Dissociation of neural tissues using the MACS® Neural Tissue Dissociation Kit Figure 2 Antigen compatibility of Neural Tissue Dissociation Kits. The table shows the recommended kit regarding antigen compatibility for subsequent MACS® Cell Separation. For epitopes not yet specified in the table, the initial use of the Neural Tissue Dissociation Kit (P) is recommended. Epitope sensitivities have been tested for antibodies available from Miltenyi Biotec. Figure 3 Tissues from various species tested with the Neural Tissue Dissociation Kits.