gentleMACS™ Dissociator
Description The gentleMACS™ Dissociator is a benchtop instrument for the automated dissociation of tissues. Two types of unique gentleMACS Tubes used with the instrument enable the time-saving and easy dissociation or homogenization of tissues in a closed system. C Tubes are used for the gentle preparation of single-cell suspensions from tissues, whereas M Tubes are used for the homogenization of tissues or cells. A single sample or two samples in parallel can be processed. The instrument offers optimized gentleMACS Programs for a variety of specific applications. The standardized tissue dissociation or homogenization procedures make for reliable and reproducible results. The gentleMACS Dissociator: Time-saving automated tissue dissociation Standardized procedure High level of user safety Sterile sample handling Applications The gentleMACS Dissociator in combination with C Tubes is designed for the preparation of single-cell suspensions from various tissues. The tissue dissociation procedure is gentle to cells and yields cell suspensions with a high viability rate. Specific gentleMACS Programs and Protocols are available for the dissociation of various tissues. Dissociation of mouse spleen Dissociation of mouse spleen using Collagenase D Dissociation of mouse lung using Collagenase D 2,3,4,5,7 Dissociation of mouse liver using Collagenase IV Dissociation of mouse heart Dissociation of implanted mouse tumors10 NEW: Dissociation of human tumor tissue12 Dissociation of mouse brain13 in combination with MACS® Neural Tissue Dissociation Kits The gentleMACS Dissociator has also been used for the dissociation of synovial membranes1, for the preparation of murine thymic epithelial cells, endothelial cells and DCs6 and for the isolation of mouse kidney tubules14. Homogenization of tissues to extract biomolecules for molecular analyses is performed using M Tubes.8,9 gentleMACS Programs and Protocols for the fast and thorough tissue homogenization are available for the isolation of total RNA11, mRNA or for the extraction of proteins. Miltenyi Biotec continuously expands the range of specific applications for the gentleMACS Dissociator. Watch out for new gentleMACS Programs and Protocols. Further information gentleMACS Protocol overview [PDF; 108,6 KB] gentleMACS Dissociator flyer [PDF; 109,7 KB] gentleMACS Dissociator user manual, version 4 [PDF; 415,4 KB] gentleMACS Dissociator – reference list [PDF; 71,4 KB] Dissociation of tumor tissue and magnetic isolation of tumor cell subpopulations [PDF; 2,4 MB]		Figure 1 genteMACS NEWS New protocols: Preparation of single-cell suspensions from human tumors Homogenization of tissues for protein extraction Upgrade your gentleMACS Instrument with the new programs! Sign in for demo Figure 2 More videos here... Figure 3 The gentleMACS™ Dissociator, shown with M Tubes and C Tubes Figure 4 Introductory video Watch the short educational video to learn how the gentleMACS Dissociator can make your work easier.

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