MACS® Supplement B27 PLUS ( #130-093-566 )

MACS® Supplement B27 PLUS

Overview

MACS® Supplement B27 PLUS is a serum-free supplement, based on B27, and designed for use in combination with MACS Neuro Medium. It is a re-optimized formulation of the B27 components designed to improve performance¹. It has been developed for the in vitro propagation of neural cells of the central and peripheral nervous system.

Details

B27 PLUS supplement has been designed for

optimal growth and long term viability of neural cells of the central and peripheral nervous system
the growth of nearly pure populations of neural cells without the need of an astrocyte feeder layer

Figure 1

Light microscopic picture of neurosphere formation after magnetic cell sorting using Anti-Prominin-1 MicroBeads after 7 days of cultivation in MACS Neuro Medium supplemented with B27 PLUS supplement. Cells were prepared from CD1 mouse brain (P3) using the Neural Tissue Dissociation
Kit (P).

Prominin-1–positive neurospheres

Figure 2

Light microscopic picture of PSA-NCAM–positive neuronal precursor cells.
After 7 days of cultivation in MACS Neuro Medium supplemented with B27 PLUS supplement. PSA-NCAM–positive cells were processed for immunofluorescent detection of anti-GAD67 antibody and goat anti mouse AF-594 (red), a protein marker for neuronal precursor cells. Cells were prepared from CD1 mouse brain (P3) using the Neural Tissue Dissociation Kit (T).

PSA-NCAM–positive neuronal precursor cells

Figure 3

Light microscopic picture of A2B5-positive glial precurosr cells.
After 7 days of cultivation in MACS Neuro Medium supplemented with B27 PLUS supplement. A2B5-positive cells were processed for
immunofluorescent detection of anti-GFAP antibody and goat anti rabbit AF-488 (green), a protein marker for glial precursor cells.
Cells were prepared from CD1 mouse brain (P3) using the Neural Tissue Dissociation Kit (T).

A2B5-positive glial precursor cells

Light microscopic picture of A2B5-positive glial precurosr cells.<br>After 7 days of cultivation in MACS Neuro Medium supplemented with B27 PLUS supplement. A2B5-positive cells were processed for<br>immunofluorescent detection of anti-GFAP antibody and goat anti rabbit AF-488 (green), a protein marker for glial precursor cells.<br>Cells were prepared from CD1 mouse brain (P3) using the Neural Tissue Dissociation Kit (T).