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| Overview |
| MACS Neuro Medium is a serum-free cell culture medium, which promotes optimum growth and long-term survival of cells from the mouse, rat, or human central or peripheral nervous system (CNS or PNS, respectively).¹,² |
| Details |
| MACS Neuro Medium has been optimized for the culture of neural cells after tissue dissociation using Neural Tissue Dissociation Kits and subsequent cell isolation using magnetic cell sorting. Supplemented with the appropriate growth factors and supplements, MACS Neuro Medium is suitable for the formation of neurospheres (neurosphere assay) from magnetically isolated neural progenitor/ stem cells as well as for the culture of magnetically sorted neuronal and glial precursor or differentiated cells from various tissues of the developing postnatal and adult nervous system. MACS Supplement B27 PLUS is recommended for low-density plating, long-term viability, and growth of CNS and PNS neural cells. |
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| Figure 1 |
| Light microscopic picture of neurosphere formation after 7 days of cultivation in MACS Neuro Medium supplemented with B27 supplement. Cells were prepared from CD1 mouse brain (P3) using the Neural Tissue Dissociation Kit (P) and magnetically isolated using Anti-Prominin-1 MicroBeads prior to cell culture. |
| Prominin-1–positive neurospheres |
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| Figure 2 |
| Light microscopic picture of PSA-NCAM–positive neuronal precursor cells after 7 days of cultivation in MACS Neuro Medium supplemented with B27 supplement. PSA-NCAM–positive cells were processed for immunofluorescent detection of anti-GAD67 antibody and goat anti mouse AF-594 (red), a marker for neuronal precursor cells. Cells were prepared from CD1 mouse brain (P3) using the Neural Tissue Dissociation Kit (T). |
| PSA-NCAM–positive neuronal precursor cells |
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| Figure 3 |
Light microscopic picture of A2B5-positive glial precursor cells after 7 days of cultivation in MACS Neuro Medium supplemented with B27 supplement. A2B5-positive cells were processed for immunofluorescent detection of anti-GFAP antibody and goat anti rabbit AF-488 (green), a marker for glial precursor cells.
Cells were prepared from CD1 mouse brain (P3) using the Neural Tissue Dissociation Kit (T). |
| A2B5-positive glial precursor cells |
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| Details |
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| References |
| 1. Brewer, G.J. et al. (1993) J. Neurosci.Res. 35: 567–576. |
| 2. Brewer, G.J. (1997) J. Neurosci. Methods 71: 143–155. |
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