MACS® Neuro Medium
Description MACS Neuro Medium has been optimized for the culture of neural cells after tissue dissociation using Neural Tissue Dissociation Kits and subsequent cell isolation using magnetic cell sorting. MACS Neuro Medium is a serum-free cell culture medium, which promotes optimum growth and long-term survival of cells from the mouse, rat, or human central or peripheral nervous system (CNS or PNS, respectively).¹,² Supplemented with the appropriate growth factors and supplements, MACS Neuro Medium is suitable for the formation of neurospheres (neurosphere assay) from magnetically isolated neural progenitor/ stem cells as well as for the culture of magnetically sorted neuronal and glial precursor or differentiated cells from various tissues of the developing postnatal and adult nervous system. MACS Supplement B27 PLUS (#130-093-566) is recommended for low-density plating, long-term viability, and growth of CNS and PNS neural cells. Applications Propagation of neural cells of the central and peripheral nervous system isolated by MACS Technology Further information Neuroscience research brochure [PDF; 1,9 MB]		Figure 1 Light microscopic picture of neurosphere formation after magnetic cell sorting using Anti-Prominin-1 MicroBeads after 7 days of cultivation in MACS Neuro Medium supplemented with B27 supplement. Cells were prepared from CD1 mouse brain (P3) using the Neural Tissue Dissociation Kit (P). Prominin-1–positive neurospheres Figure 2 Light microscopic picture of PSA-NCAM–positive neuronal precursor cells after 7 days of cultivation in MACS Neuro Medium supplemented with B27 supplement. PSA-NCAM–positive cells were processed for immunofluorescent detection of anti-GAD67 antibody and goat anti mouse AF-594 (red), a marker for neuronal precursor cells. Cells were prepared from CD1 mouse brain (P3) using the Neural Tissue Dissociation Kit (T). PSA-NCAM–positive neuronal precursor cells Figure 3 Light microscopic picture of A2B5-positive glial precursor cells after 7 days of cultivation in MACS Neuro Medium supplemented with B27 supplement. A2B5-positive cells were processed for immunofluorescent detection of anti-GFAP antibody and goat anti rabbit AF-488 (green), a marker for glial precursor cells. Cells were prepared from CD1 mouse brain (P3) using the Neural Tissue Dissociation Kit (T). A2B5-positive glial precursor cells

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