MACS® Neuro Medium ( #130-093-570 )

MACS^® Neuro Medium

Description

MACS Neuro Medium has been optimized for the culture of neural cells after tissue dissociation using Neural Tissue Dissociation Kits and subsequent cell isolation using magnetic cell sorting. MACS Neuro Medium is a serum-free cell culture medium, which promotes optimum growth and long-term survival of cells from the mouse, rat, or human central or peripheral nervous system (CNS or PNS, respectively).¹^,² Supplemented with the appropriate growth factors and supplements, MACS Neuro Medium is suitable for the formation of neurospheres (neurosphere assay) from magnetically isolated neural progenitor/ stem cells as well as for the culture of magnetically sorted neuronal and glial precursor or differentiated cells from various tissues of the developing postnatal and adult nervous system.

Applications

Propagation of neural cells of the central and peripheral nervous system isolated by MACS Technology

Further information

Neuroscience research brochure
[PDF; 1,9 MB]

Figure 1

Light microscopic picture of neurosphere formation after magnetic cell sorting using Anti-Prominin-1 MicroBeads after 7 days of cultivation in MACS Neuro Medium supplemented with B27 supplement. Cells were prepared from CD1 mouse brain (P3) using the Neural Tissue Dissociation
Kit (P).

Prominin-1–positive neurospheres

Figure 2

Light microscopic picture of PSA-NCAM–positive neuronal precursor cells.
After 7 days of cultivation in MACS Neuro Medium supplemented with B27 supplement. PSA-NCAM–positive cells were processed for immunofluorescent detection of anti-GAD67 antibody and goat anti mouse AF-594 (red), a protein marker for neuronal precursor cells. Cells were prepared from CD1 mouse brain (P3) using the Neural Tissue Dissociation Kit (T).

PSA-NCAM–positive neuronal precursor cells

Figure 3

Light microscopic picture of A2B5-positive glial precurosr cells.
After 7 days of cultivation in MACS Neuro Medium supplemented with B27 supplement. A2B5-positive cells were processed for
immunofluorescent detection of anti-GFAP antibody and goat anti rabbit AF-488 (green), a protein marker for glial precursor cells.
Cells were prepared from CD1 mouse brain (P3) using the Neural Tissue Dissociation Kit (T).

A2B5-positive glial precursor cells

Light microscopic picture of A2B5-positive glial precurosr cells.<br>After 7 days of cultivation in MACS Neuro Medium supplemented with B27 supplement. A2B5-positive cells were processed for<br>immunofluorescent detection of anti-GFAP antibody and goat anti rabbit AF-488 (green), a protein marker for glial precursor cells.<br>Cells were prepared from CD1 mouse brain (P3) using the Neural Tissue Dissociation Kit (T).