PepTivator - EBV EBNA-1
Description The PepTivator – EBV EBNA-1 is a peptide pool that consists mainly of 15-mer peptides with 11 amino acids overlap. It has been especially developed for efficient in vitro stimulation of EBV EBNA-1–specific CD4+ and CD8+ T cells as peptides of 15-aa length with 11 aa overlap represent an optimized solution for stimulating both CD4+ and CD8+ T cells in various applications.1 Epstein-Barr virus (EBV) is a human γ-herpes virus with B cell growth–transforming ability and lymphomagenic potential. More than 90% of human adults are infected with EBV. Usually the primary infection is asymptomatic, but some individuals develop an infectious mononucleosis, a self-limiting lymphoproliferative disease. The Epstein-Barr nuclear antigen 1 (EBNA-1) protein is involved in the replication of viral episomes and therefore crucial for the persistence of the infection. Its expression is maintained in all latency cycles of infection and in all EBV-associated malignancies. EBNA-1-specific CD4+ and CD8+ T cells have been found in EBV-infected individuals.2–8 Applications Quantitative, phenotypical, or functional analysis of EBV EBNA-1–specific T cell immunity can provide important information on the natural course of immune responses in healthy or immunocompromised individuals. The in vitro stimulation of EBV EBNA-1–specific CD4+ and CD8+ T cells with PepTivator – EBV EBNA-1 causes the secretion of effector cytokines and the upregulation of activation markers, such as CD154 or CD137. These cytokines or activation markers then allow the detection and isolation of EBV EBNA-1–specific T cells: Detection and analysis of EBV EBNA-1–specific CD4+ and CD8+ effector/memory T cells in PBMCs by MACS® Cytokine Secretion Assays, intracellular cytokine staining, or other technologies Isolation of viable EBV EBNA-1–specific CD4+ T cells with the CD154 MicroBead Kit, or of CD4+ and CD8+ T cells using the CD137 MicroBead Kit or MACS Cytokine Secretion Assay – Cell Enrichment and Detection Kits. Subsequently, cells can be expanded for generation of T cell lines for research on immunotherapy of EBV infection. Generation of EBV EBNA-1–specific CD4+ and CD8+ effector/memory T cells from naive T cell populations for research on immunotherapy and vaccination PepTivator – EBV EBNA-1 can also be used for pulsing of antigen-presenting cells, for example, for research on dendritic cell vaccination.

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