|
|
 |
|
| Description |
The PepTivator - CMV IE-1 is a peptide pool that consists mainly of 15-mer peptides with 11–amino acid (aa) overlap, covering the complete sequence of the IE-1 protein of human cytomegalovirus (CMV).
CMV IE-1 (immediate early protein 1), also known as UL123, is a non-structural protein, one of the first CMV antigens expressed in an infected cell, and predominantly induces a CD8+ T cell response.
IE-1-specific T cells occur in infected individuals at frequencies comparable to those of pp65-specific CD8+ T cells.1 Both CMV antigens, IE-1 and pp65, are considered dominant T cell targets.
The PepTivator - CMV IE-1 has been especially developed for efficient in vitro stimulation of CMV IE-1-specific CD4+ and CD8+ T cells, as peptides of 15 aa length with 11-aa overlap represent an optimized solution for stimulating both CD4+ and CD8+ T cells in various applications.2 |
| Applications |
Quantitative, phenotypical, or functional analysis of IE-1-specific T cells can provide important information on the natural course of immune responses in healthy or immunocompromised individuals.
The in vitro stimulation of IE-1-specific CD4+ and CD8+ T cells with PepTivator - CMV IE-1 causes the secretion of effector cytokines and the upregulation of activation markers, such as CD154 or CD137. These cytokines or activation markers then allow the detection and isolation of IE-1-specific T cells, for example:
- Detection and analysis of CMV IE-1-specific CD4+ and CD8+ effector/memory T cells in PBMCs by MACS® Cytokine Secretion Assays, intracellular staining, or other technologies.
- Isolation of viable CMV IE-1-specific CD4+ T cells with the CD154 MicroBead Kit, or of CD4+ and CD8+ T cells using the CD137 MicroBead Kit or MACS Cytokine Secretion Assay - Cell Enrichment and Detection Kits. Subsequently, cells can be expanded for generation of T cell lines for research on immunotherapy of CMV infection.
- Generation of CMV IE-1-specific CD4+ and CD8+ effector/memory T cells from naive T cell populations for research on immunotherapy and vaccination.
PepTivator - CMV IE-1 can also be used for pulsing of antigen-presenting cells for research on dendritic cell vaccination. |
| |
| Figure 1 |
| From a CMV+ donor 106 human PBMCs were restimulated for 6 hours with 20 ÎĽL/mL of reconstituted PepTivator - CMV IE-1 or incubated without antigen. After 2 hours 1 ÎĽg/mL brefeldin A was added. Cells were fixed, permeabilized, and intracellularly stained with Anti-IFN-Îł-PE. T cells were counterstained for CD8 expression. |
|
| Stimulated sample |
 |
| Unstimulated control |
 |
|
|
|
|
|
| My favorites |
| Your current favorite list is empty. |
| Products |
| PepTivator - CMV IE-1, human |
- 6 nmol/peptide for stimulation of 108 cells
Download data sheet
130-093-493
|
- 60 nmol/peptide for stimulation of 109 cells
Download data sheet
130-093-494
|
|
| MACS References |
| 1. Kern et al. (1999) J. Virol. 73: 8179–8184. |
| 2. Kiecker et al. (2004) Hum. Immunol. 65: 523–536. |
|
|