PepTivator - CMV pp65
Description The PepTivator - CMV pp65 is a peptide pool that consists mainly of 15-mer peptides with 11 amino acid (aa) overlap, covering the complete sequence of the pp65 protein of human cytomegalovirus (CMV). CMV pp65 (65 kDa lower matrix phosphoprotein), also known as glycoprotein 64 or UL83, is a virion tegument protein and the main component of the enveloped subviral particle. CMV pp65 is an immunodominant target of CD4+ and CD8+ T cell responses to CMV. CMV pp65-specific T cells predominantly produce inflammatory cytokines, such as IFN-γ, IL-2, and TNF-α. The PepTivator - CMV pp65 has been especially developed for efficient in vitro stimulation of CMV pp65-specific CD4+ and CD8+ T cells, as peptides of 15 aa length with 11 aa overlap represent an optimized solution for stimulating both CD4+ and CD8+ T cells in various applications. Applications Quantitative, phenotypical, or functional analysis of pp65-specific T cell immunity can provide important information on the natural course of immune responses in healthy or immunocompromized individuals. The in vitro stimulation of pp65-specific CD4+ and CD8+ T cells with PepTivator - CMV pp65 causes the secretion of effector cytokines and the upregulation of activation markers, such as CD154 or CD137. These cytokines or activation markers then allow the detection and isolation of pp65-specific T cells: - Detection and analysis of CMV pp65-specific CD4+ and CD8+ effector/memory T cells in PBMCs by MACS® Cytokine Secretion Assays, intracellular staining, or other technologies. - Isolation of viable CMV pp65-specific CD4+ T cells with the CD154 MicroBead Kit, or of CD4+ and CD8+ T cells using the CD137 MicroBead Kit or MACS Cytokine Secretion Assay - Cell Enrichment and Detection Kits. Subsequently, cells can be expanded for generation of T cell lines for research on immunotherapy of CMV infection. - Generation of CMV pp65-specific CD4+ and CD8+ effector/memory T cells from naive T cell populations for research on immunotherapy and vaccination. PepTivator - CMV pp65 can also be used for pulsing of antigen-presenting cells for research on dendritic cell vaccination.		Figure 1 107 human PBMCs of a CMV+ donor were restimulated for 4 hours with 20 μL/mL of reconstituted PepTivator - CMV pp65. pp65-specific cells were stained and magnetically enriched according to their secretion of IFN-γ using the IFN-γ Secretion Assay - Cell Enrichment and Detection Kit. T cells were counterstained for CD4 and CD8 expression. Cell debris and dead cells were excluded from the analysis based on scatter signals and PI fluorescence. IFN-γ secretion of viable lymphocytes is shown. CD4+ T cells After enrichment Before enrichment CD8+ T cells Before enrichment After enrichment