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| PepTivator® JCV VP3 – research grade |
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| Description |
PepTivator® JCV VP3 – research grade is a pool of lyophilized peptides, consisting mainly of 15-mer sequences with 11 amino acids overlap, covering the complete sequence of the human JC virus (JCV) VP3 protein.
In vitro stimulation of antigen-specific T cells with PepTivator Peptide Pools causes the secretion of effector cytokines and the up-regulation of activation markers, which then allow the detection and isolation of antigen-specific T cells. |
| Details |
Background information
JCV is a widespread human polyomavirus. Primary infection results in asymptomatic, latent persistence of the virus. Immunodeficiency or immunosuppression allows virus reactivation. VP3 is the minor capsid protein expressed late in the lytic cycle.
Downstream applications
PepTivator JCV VP3 has been specifically developed for efficient in vitro stimulation of JCV-specific T cells. Peptides of 15 amino acids in length and 11 amino acids overlap represent an optimized solution for stimulating both CD4+ and CD8+ T cells in various applications, including:
- Detection and analysis of JCV VP3–specific effector/memory T cells in PBMCs by MACS® Cytokine Secretion Assays, intracellular cytokine staining, or other technologies.
- Isolation of viable JCV VP3–specific CD4+ T cells with the CD154 MicroBead Kit, or of CD4+ and CD8+ T cells using the CD137 MicroBead Kit or MACS Cytokine Secretion Assay – Cell Enrichment and Detection Kits. Subsequently, cells can be expanded for generation of T cell lines.
- Generation of JCV VP3–specific effector/memory T cells from naive T cell populations.
- Pulsing of antigen-presenting cells.
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| Details |
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| Products |
| PepTivator JCV VP3 – research grade, human |
- 6 nmol/peptide for stimulation of 108 cells
Download datasheet
130-096-762
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