PepTivator® HPV18 E7 ( #130-095-996 #130-096-007 )

PepTivator® HPV18 E7

Description

PepTivator^® HPV18 E7 is a peptide pool that consists mainly of 15-mer peptides with 11–amino acids (aa) overlap, covering the complete sequence of the human papillomavirus 18E7 protein.
PepTivator peptide pools have been developed for the efficient in vitro stimulation of antigen–specific T cells, as peptides of 15 amino acids in length and 11 amino acids overlap represent an optimized solution for stimulating both CD4⁺ and CD8⁺ T cells in various applications.
Quantitative, phenotypical, or functional analysis of HPV18 E7–specific T cell immunity can provide important information on the natural course of immune responses in healthy or immunocompromised individuals.

Details

Background information
Human papillomaviruses (HPV) are non-enveloped, doublestranded circular DNA viruses causing many different infections. Persistent infections with several HPV types are associated with the development of tumors. HPV types 16 and 18 together cause about 70 percent of cervical cancers. The viral E6 and E7 proteins of HPV16 and HPV18 are involved in the initiation and maintenance of oncogenic processes. HPV E6 and E7-specific T cell immune responses play an important role in successfully clearing HPV infection and controlling HPV-associated diseases.

Downstream applications
The in vitro stimulation of antigen-specific T cells with PepTivator HPV18 E7 causes the secretion of effector cytokines and the up-regulation of activation markers, such as CD154 or CD137. These cytokines or activation markers then allow the detection and isolation of HPV18 E7–specific T cells.

Detection and analysis of HPV18 E7–specific CD4⁺ and CD8⁺ effector/memory T cells in PBMCs by MACS® Cytokine Secretion Assays, intracellular cytokine staining, or other technologies.
Isolation of viable HPV18 E7–specific CD4⁺ T cells with the CD154 MicroBead Kit, or of CD4⁺ and CD8⁺ T cells using the CD137 MicroBead Kit or MACS Cytokine Secretion Assay – Cell Enrichment and Detection Kits. Subsequently, cells can be expanded for generation of T cell lines.
Generation of HPV18E7–specific CD4⁺ and CD8⁺ effector/memory T cells from naive T cell populations for research on immunotherapy and vaccination.
Pulsing of antigen-presenting cells for research on dendritic cell vaccination.