PepTivator® NY-ESO-1 ( #130-095-380 #130-095-381 )

PepTivator® NY-ESO-1

Description

PepTivator® NY-ESO-1 is a pool of lyophilized peptides, consisting mainly of 15-mer sequences with 11 amino acids overlap, covering the complete sequence of the human NY-ESO-1 protein.
PepTivator peptide pools have been developed for the efficient in vitro stimulation of antigen–specific CD4⁺ and CD8⁺ T cells as peptides of 15-aa length with 11-aa overlap represent an optimized solution for stimulating both CD4⁺ and CD8⁺ T cells in various applications.
Quantitative, phenotypical, or functional analysis of NY-ESO-1–specific T cell immunity can provide important information on the natural course of immune responses.

Details

Background information
NY-ESO-1 belongs to the cancer-testis family of antigens. It is highly expressed in male germ cells but generally not in other normal tissues. Aberrant expression is found in a variable proportion of a wide range of different cancer types. Patients with advanced NY-ESO-1-expressing tumors frequently develop humoral and cellular immunity to NY-ESO-11. NY-ESO is an attractive antigen to monitor anti-tumor immune responses and is used for immunotherapeutic interventions.

Downstream applications
The in vitro stimulation of NY-ESO-1–specific CD4⁺ and CD8⁺ T cells with the PepTivator NY-ESO-1 peptide pool causes the secretion of effector cytokines and the up-regulation of activation markers, such as CD154 or CD137. These cytokines or activation markers then allow the detection and isolation of NY-ESO-1–specific T cells.

Detection and analysis of specific CD4⁺ and CD8⁺ effector/memory T cells in PBMCs by MACS^® Cytokine Secretion Assays, intracellular cytokine staining, or other technologies
Isolation of viable tyrosinase–specific CD4⁺ T cells with the CD154 MicroBead Kit, or of CD4⁺ and CD8⁺ T cells using the CD137 MicroBead Kit or MACS Cytokine Secretion Assay – Cell Enrichment and Detection Kits. Subsequently, cells can be expanded for generation of T cell lines.
Generation of tyrosinase–specific CD4⁺ and CD8⁺ effector/memory T cells for research on immunotherapy and vaccination
Pulsing of antigen-presenting cells.