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| PepTivator® gp100/Pmel 17 |
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| Description |
The PepTivator® gp100/Pmel 17 is a peptide pool that consists mainly of 15-mer peptides with 11–amino acids (aa) overlap, covering the complete sequence of the antigen.
PepTivator peptide pools have been developed for the efficient in vitro stimulation of antigen–specific CD4+ and CD8+ T cells, as peptides of 15-aa length with 11-aa overlap represent an optimized solution for stimulating both CD4+ and CD8+ T cells in various applications.1
Quantitative, phenotypical, or functional analysis of gp100/Pmel 17–specific T cell immunity can provide important information on the natural course of immune responses. |
| Details |
Background information
Gp100/Pmel 17 belongs to the melanoma-associated antigens of the group of differentiation antigens. Other members of this group are, for example, tyrosinase and Melan-A/MART-1. Gp100/Pmel 17 is expressed on normal melanocytes, but is also present in melanoma. CD4+ and CD8+ T lymphocytes recognizing gp100/Pmel 17 have been identified in melanoma patients.2
Downstream applications
The in vitro stimulation of gp100/Pmel 17 CD4+ and CD8+ T cells with PepTivator gp100/Pmel 17 causes secretion of effector cytokines and upregulation of activation markers, which then allow the detection and isolation of gp100/Pmel 17–specific T cells.
- Detection and analysis of gp100/Pmel 17–specific CD4+ and CD8+ effector/memory T cells in PBMCs by MACS® Cytokine Secretion Assays, intracellular cytokine staining, or other technologies
- Isolation of viable gp100/Pmel 17–specific CD4+ T cells with the CD154 MicroBead Kit, or of CD4+ and CD8+ T cells using the CD137 MicroBead Kit or MACS Cytokine Secretion Assay – Cell Enrichment and Detection Kits. Subsequently, cells can be expanded for generation of T cell lines.
- Generation of gp100/Pmel 17–specific CD4+ and CD8+ effector/memory T cells for research on immunotherapy and vaccination
- Pulsing of antigen-presenting cells, for example, for research in dendritic cell vacchination..
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| Details |
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| Products |
| PepTivator gp100/Pmel 17, human |
- 6 nmol/peptide for stimulation of 108 cells
Download datasheet
130-094-449
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- 60 nmol/peptide for stimulation of 109 cells
Download datasheet
130-094-450
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| References |
| 1. Kiecker et al. (2004) Hum. Immunol. 65: 523–536. |
| 2. Boon et al. (2006) Annu. Rev. Immunol. 24:175–208. |
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