PepTivator® Melan-A/MART-1 ( #130-094-477 #130-094-597 )

PepTivator® Melan-A/MART-1

Description

PepTivator^® Melan-A/MART-1 is a peptide pool that consists mainly of 15-mer peptides with 11–amino acid (aa) overlap, covering the complete sequence of the human MelanA/MART-1 protein.
PepTivator peptide pools have been developed for the efficient in vitro stimulation of antigen–specific CD4⁺ and CD8⁺ T cells as peptides of 15-aa length with 11-aa overlap represent an optimized solution for stimulating both CD4⁺ and CD8⁺ T cells in various applications.¹
Quantitative, phenotypical, or functional analysis of Melan-A/MART-1–specific T cell immunity can provide important information on the natural course of immune responses.

Details

Background information
Melanoma antigen recognized by T cells (MART-1) belongs to the tumor-associated antigens of the group of differentiation antigens. Its expression is restricted to normal melanocytes, but it is also present in melanoma. CD4⁺ and CD8⁺ T lymphocytes recognizing Melan-A/MART-1 have been identified in melanoma patients. Therefore, Melan-A/MART-1 may represent a potential target for immunotherapy of melanoma.²

Downstream applications
The in vitro stimulation of Melan-A/MART-1–specific CD4⁺ and CD8⁺ T cells with PepTivator Melan-A/MART-1 causes the secretion of effector cytokines and the upregulation of activation markers, such as CD154 or CD137. These cytokines or activation markers then allow the detection and isolation of Melan-A/MART-1 –specific T cells.

Detection and analysis of Melan-A/MART-1 –specific CD4⁺and CD8⁺ effector/memory T cells in PBMCs by MACS® Cytokine Secretion Assays, intracellular cytokine staining, or other technologies
Isolation of viable Melan-A/MART-1–specific CD4⁺ T cells with the CD154 MicroBead Kit, or of CD4⁺ and CD8⁺ T cells using the CD137 MicroBead Kit or MACS Cytokine Secretion Assay – Cell Enrichment and Detection Kits. Subsequently, cells can be expanded for generation of T cell lines.
Generation of Melan-A/MART-1–specific CD4⁺ and CD8⁺ effector/memory T cells from naive T cell populations for research on immunotherapy and vaccination.
Pulsing of antigen-presenting cells.