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| Description |
| Anti-A2B5 MicroBeads have been developed for the isolation of A2B5+ cells. The Anti-A2B5 MicroBeads recognize the c-series ganglioside–specific antigen A2B5. Glycosphingolipids (GSLs) including gangliosides are considered to be useful cell-surface markers for monitoring neural stem and precursor cells and their progeny, and have been utilized as specific markers to define oligodendrocyte lineage cells during development. The first GSL antigen expressed in an oligodendrocyte lineage is the A2B5 antigen. A2B5 is a monoclonal antibody 1, where the antigens recognized were established as the c-series gangliosides including GQ1c, GT1c, and GT3 2,3. A2B5 is predominantly expressed in embryonic and neonatal neural tissue. Glial precursor cells are defined as cells that give rise to glial cell types, such as astrocytes and oligodendrocytes, and during development, A2B5 antigens are expressed in glial precursor cells, known as O-2A progenitor cells, which differentiate into type-2 astrocytes and oligodendrocytes 4. These antigens are expressed not only in O-2A progenitor cells but also in fetal glial precursor cells 5. In adult mammalian brain A2B5 expression is restricted mainly to areas that retain neurogenic potential such as the subventricular zone (SVZ). Ganglioside GT3 and its O-acetylated derivative are the principal A2B5-reactive gangliosides, and both antigens are down-regulated as the cells differentiate into mature oligodendrocytes. Thus, A2B5 is considered as a marker for immature glial committed precursor cells, and since the A2B5 antigens are localized on the cell surface, the antibody can be used to isolate glial progenitors from embryonic to adult human, murine, and rat tissue by immunomagnetic isolation according to the expression of A2B5.6–13 |
| Applications |
Isolation of cells expressing A2B5.
Positive selection of glial progenitor cells from human, murine, and rat embryonic to adult tissue.6-13 |
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| Figure |
| Isolation of A2B5+ cells from day 1 postnatal mouse using Neural Tissue Dissociation Kit (P), an MS-Column, FcR Blocking Reagent, mouse (1:10). |
| Dissociated brain cells before separation |
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| A2B5– cells |
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| A2B5+ cells |
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| MACS References |
| 1. Eisenbarth et al (1979) Proc Natl Acad Sci USA. 76: 4913-4917 |
| 2. Kasai et al, (1983) Brain Res. 277 : 155-158 |
| 3. Saito et al (2001) J. Neurochem 78 : 64-74 |
| 4. Raff et al (1983) Nature 303 : 390-396 |
| 5. Dietrich et al (2002) Glia 40 : 65-77 |
| 6. Windrem et al. (2004) Nat. Med. 10: 93–97. |
| 7. Ruffini et al. (2004) Am. J. Pathol. 165: 2167–2175. |
| 8. Larsen et al. (2006) J. Neurosci. 26: 2207–2214. |
| 9. Seidenfaden et al (2006) Mol Cell Neurosci. 32 : 187-198 |
| 10. Larsen et al. (2004) J. Neurosci. 24: 7597–7603. |
| 11. Liu et al (2007) J. Neurosci. 27: 7339-7343 |
| 12. Larsen et al. (2003) J. Neurosci. 23: 11127–11135. |
| 13. Strathman et al (2007) BMC Developmental Biology 7: 33 |
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