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| Overview |
| Anti-PSA-NCAM MicroBeads have been developed for the positive selection or depletion of PSA-NCAM+ cells from tissue or cell culture. |
| Details |
Background information
Anti-PSA-NCAM MicroBeads recognize polysialic acid (PSA), which is linked to the extracellular domain of the neural cell adhesion molecule (NCAM, CD56).1 PSA-NCAM, the highly polysialated form of NCAM, is predominantly expressed in embryonic and neonatal neural tissue2. In adult mammalian brain PSA-NCAM expression is restricted mainly to areas that retain neurogenic potential, such as the subventricular zone (SVZ)3 and the dentate gyrus of the hippocampus.4 PSA-NCAM is a marker for immature neuronal-committed progenitors that are permanently generated in the SVZ and migrate along a well-defined pathway, the rostral migratory stream, into the olfactory bulb where they differentiate into GABAergic and dopaminergic interneurons.3,5 PSA-NCAM positive neuronal precursors have been isolated from rat forebrain and from mouse SVZ tissue after depletion of A2B5+ glial progenitor cells.6-10
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| Figure 1 |
| Separation of a single-cell suspension derived from P1 mouse whole-brain tissue using the Neural Tissue Dissociation Kit (T), Anti-PSA-NCAM MicroBeads, a MiniMACS™ Separator, and an MS Column. Cells were fluorescently stained with rat anti-mouse IgM-APC and analyzed by flow cytometry. |
| A: Neural cells before separation |
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| B: PSA-NCAM- cells |
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| C: PSA-NCAM+ cells |
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| Details |
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| Products |
| Anti-PSA-NCAM MicroBeads, human, mouse, rat |
- for 1×109 total cells
Download datasheet
130-092-966
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| References |
| 1. Rougon, G. and Marshak, D.R. (1986) J. Biol. Chem. 261: 3396–3401. |
| 2. Kiss, J.Z. and Muller, D. (2001) Rev. Neurosci. 12: 297–310. |
| 3. Doetsch et al. (1997) J. Neurosci. 17: 5046–5061. |
| 4. Seki (2002) J. Neurosci. Res. 70: 327–334. |
| 5. Pennartz et al. (2004) Moll. Cell. Neurosci. 25: 692–706. |
| 6. Seidenfaden et al. (2006) Mol. Cell. Neurosci. 32: 187–98. |
| 7. Seidenfaden et al. (2006) MACS&more 10 (1): 4–6. |
| 8. Marmur et al. (1998) J. Neurosci. 18: 9800–9811. |
| 9. Strathmann et al. (2007) BMC Dev. Biol. 7: 33. |
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