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| Retinal Ganglion Cell Isolation Kit |
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| Overview |
| These kits and tool boxes have been developed for the isolation of rat retinal ganglion cells (RGCs). |
| Details |
Background information
Retinal ganglion cells (RGCs) are neurons of the central nervous system, which transmit information from the retina to the visual center of the brain. RGCs have been used to study neuron-glia interactions and the mechanisms of neurodegeneration.1–4
Detailed separation procedure
The separation procedure involves the depletion of endothelial cells and microglia using MACSiBead™ Particles, and the positive selection of CD90.1+ RGCs using CD90.1 MicroBeads.5
First, the cell suspension is incubated with a biotin-conjugated antibody, which binds to endothelial cells and microglia, and with CD90.1 MicroBeads labeling RGCs. In the next step, endothelial cells and microglia are magnetically labeled with Anti-Biotin MACSiBead Particles and depleted by using a MACSiMAG™ Separator. Subsequently, the cell fraction depleted of endothelial cells and microglia is applied to MS Columns for positive selection of the CD90.1+ RGCs.
Note: MACSiBead Particles are not suitable for separations using MACS Columns and MiniMACS™, MidiMACS™, VarioMACS™, SuperMACS™, or autoMACS® Separators. |
| Columns |
| For positive selection: MS Columns |
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| Details |
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| Products |
| Retinal Ganglion Cell Isolation Kit, rat |
- for 25 separations (1)
Download datasheet
130-096-209
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| Retinal Ganglion Cell Tool Box 1 |
Components
- Neural Tissue Dissociation Kit – Postnatal Neurons
- Retinal Ganglion Cell Isolation Kit, rat
Download datasheet #1
Download datasheet #2
130-096-464
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| Retinal Ganglion Cell Starter Kit |
Components
- Neural Tissue Dissociation Kit – Postnatal Neurons
- Retinal Ganglion Cell Isolation Kit, rat
- MiniMACS Separation Unit
- MACS MultiStand
- MACSiMAG Separator
Download datasheet #1
Download datasheet #2
130-096-463
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| Retinal Ganglion Cell Tool Box 2 |
Components
- Neural Tissue Dissociation Kit – Postnatal Neurons
- Retinal Ganglion Cell Isolation Kit, rat
- MACS Neuro Medium
- MACS Supplement B27 PLUS
- Human BDNF (10 µg)
Download datasheet #1
Download datasheet #2
130-096-462
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| (1) One separation corresponds to 10 retinas (5 rats, age P6–7) per sample. |
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| References |
| 1. Barres et al. (1988) Neuron 1: 791–803. |
| 2. Meyer-Franke et al. (1995) Neuron 15: 805–819. |
| 3. Pfrieger and Barres (1997) Science 277: 1684–1687 |
| 4. Göritz et al. (2007) Glia 55: 1108–1122. |
| 5. Pennartz et al. (2010) MACS&more 12 (2): 16-18. |
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