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| IL-13 Secretion Assay – Cell Enrichment and Detection Kit |
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| Description |
| The IL-13 Secretion Assay - Cell Enrichment and Detection Kit was developed for the sensitive detection as well as the enrichment of human IL-13-secreting cells. |
| Details |
Background information
Interleukin-13 (IL-13) is produced primarily by activated Th2 cells, but also by mast cells, NK cells, and dendritic cells. IL-13 exerts anti-inflammatory effects on monocytes and macrophages. Furthermore, it inhibits the expression of pro-inflammatory cytokines, such as IL-1β, TNF-α, IL-6, and IL-8. IL-13 has also been shown to enhance B cell proliferation and to induce isotype switching, resulting in an increased production of IgE.
IL-13, in contrast to IL-4, fails to induce Th2 cell differentiation, one of the hallmarks of the allergic response. |
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| Figure 1 |
| PBMCs were stimulated with CytoStim for 4 hours or left untreated. Both samples were treated with a CD40 antibody to allow the surface detection of CD154. Subsequently, IL-13-secreting cells were enriched and analyzed for IL-13 secretion and expression of CD154 using the IL-13 Secretion Assay - Cell Enrichment and Detection Kit, and CD154-APC. The plots show stimulated and unstimulated cells before and after separation. CD154 expression and IL-13 secretion by viable CD4+ T cells is shown. |
| Stimulated sample |
| A: Before enrichment |
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| B: After enrichment |
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| Unstimulated control |
| C: Before enrichment |
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| D: After enrichment |
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| Details |
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| Products |
| IL-13 Secretion Assay – Cell Enrichment and Detection Kit (PE), human |
- for 50 tests with 107 total cells
Download datasheet
130-093-480
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