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| IFN-α Secretion Assay - Cell Enrichment and Detection Kit |
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| Description |
| The IFN-α Secretion Assay – Cell Enrichment and Detection Kit was developed for the sensitive detection as well as the enrichment of human IFN-α–secreting cells. |
| Details |
Background information
Type I interferons (IFNs), including IFN-α, are a family of cytokines that have pleiotropic functions in the immune system.1 Besides its potent antiviral activity, IFN-α exerts a variety of immunomodulatory effects, including positive and negative regulation of its own production, regulation of IL-12 and IFN-γ production, promotion of natural killer (NK) cell cytotoxicity, activation and maturation of dendritic cells, and the promotion of B cell development towards plasma cells.2,3 IFN-α is expressed predominantly by CD123+CD11c–CD303 (BDCA2)+ plasmacytoid dendritic cells, also referred to as natural IFN–producing cells. These cells, representing approximately 0.4% of total PBMCs, produce up to 1000-fold more type I IFNs than any other cell type. However, biologically relevant levels of IFN-α are also produced by other cell types, e.g., monocytes and myeloid dendritic cells.4
Downstream applications
The kit can be used for the detection and enrichment of IFN-α–secreting cells for enumeration and phenotypic analysis as well as for culturing and functional characterization. Furthermore, the stimulatory or regulatory functions of cytokine-secreting monocytes and dendritic cells can be analysed. |
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| Figure 1 |
| Human PBMCs were incubated with CpG ODN 2216 for 5 hours. Cells were stained and isolated according to secretion of IFN-α using the IFN-α Secretion Assay – Cell Enrichment and Detection Kit (PE) and CD303 (BDCA-2)-APC, and analyzed by flow cytometry. |
| A: Before enrichment |
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| B: After enrichment |
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| Details |
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| Products |
| IFN-α Secretion Assay – Cell Enrichment and Detection Kit (PE), human |
- for 50 tests with 107 total cells
Download datasheet
130-092-605
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| References |
| 1. Biron, C. A. (2001) Immunity 14: 661–664. |
| 2. Montoya, M. et al. (2002) Blood 99: 3263–3271. |
| 3. Santini, S. M. et al. (2002) J. Interferon Cytokine Res. 22: 1071–1080. |
| 4. Theofilopoulos, A. N. et al. (2005) Annu. Rev. Immunol. 23: 307–336. |
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