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| IL-12 Secretion Assay – Cell Enrichment and Detection Kit |
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| Description |
| The IL-12 Secretion Assay - Cell Enrichment and Detection Kit was developed for the sensitive detection and as well as the enrichment of human cells secreting the p70 subunit of IL-12. |
| Details |
Background information
Interleukin-12 (IL-12) is a 70-75 kDa heterodimeric cytokine composed of a 40 kDa heavy chain and a 35 kDa light chain. It is mainly produced by monocytes and macrophages, as well as dendritic cells primarily in response to stimulation with bacterial or viral antigens. IL-12 displays various effects on both NK and T cells, playing an essential role in the interaction between innate and adaptive immunity. |
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| Figure 1 |
| Human PBMCs depleted of CD14+ cells were stimulated for 4 hours with LPS (10 ng/ml) and for 2 hours with R-848 (2.5 μg/ml), or left untreated. The stimulated and unstimulated cells were stained and isolated according to the secretion of IL-12 using the IL-12 Secretion Assay - Cell Enrichment and Detection Kit. Additionally, cells were stained with CD11c-APC. |
| Stimulated sample |
| A: Before enrichment |
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| B: After enrichment |
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| Unstimulated control |
| C: Before enrichment |
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| D: After enrichment |
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| Details |
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| Products |
| IL-12 Secretion Assay – Cell Enrichment and Detection Kit (PE), human |
- for 50 tests with 107 total cells
Download datasheet
130-092-122
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