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| TNF-α Secretion Assay – Cell Enrichment and Detection Kit |
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| Description |
| The TNF-α Secretion Assay - Cell Enrichment and Detection Kit was developed for the sensitive detection as well as the enrichment of human TNF-α-secreting cells. |
| Details |
Background information
Tumor necrosis factor α (TNF-α) is secreted by activated T cells, monocytes, macrophages, NK cells, and neutrophils. TNF-α is a mediator of inflammatory immune responses and is toxic for many transformed cells, especially in combination with IFN-γ.
Downstream applications
The TNF-α Secretion Assay has been developed for detection and isolation of antigen-specific TNF-α-secreting T cells after stimulation with antigen.
The TNF-α Secretion Assay can also be used to analyze secretion of TNF-α by other leukocytes, e.g., monocytes.
The TNF-α Secretion Assay was used in combination with the IFN-γ Secretion Assay to investigate cytotoxic effects of HIV-specific T cells.1 |
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| Figure 1 |
PBMCs of a CMV+ donor were stimulated for 4 hours with CMV lysate. The responding cells were stained and isolated according to secretion of TNF-α using the TNF-α Secretion Assay - Cell Enrichment and Detection Kit.
In the stimulated sample, 9390 TNF-α-secreting CD4+ T cells were enriched per 106 CD4+ T cells by using MS Columns and a MiniMACS™ Separator. In the unstimulated control sample, no TNF-α-secreting CD4+ T cells were detected per 106 CD4+ T cells after enrichment. |
| Stimulated sample |
| A: Before enrichment |
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| B: After enrichment |
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| Unstimulated control |
| C: Before enrichment |
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| D: After enrichment |
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* Percentage represents frequency among CD4+ T cells
** Percentage represents frequency among enriched cells |
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| Details |
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| Products |
| TNF-α Secretion Assay – Cell Enrichment and Detection Kit (PE), human |
- for 50 tests with 107 total cells
Download datasheet
130-091-269
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| References |
| 1. Lichterfeld et al. (2004) Blood 104: 487-494. |
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