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| IL-10 Secretion Assay - Cell Enrichment and Detection Kit |
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| Description |
| The IL-10 Secretion Assay - Cell Enrichment and Detection Kit was developed for the sensitive detection as well as the enrichment of human IL-10-secreting cells. |
| Details |
Background information
Interleukin-10 (IL-10) is predominantly secreted by human CD4+ T cells and antigen-presenting cells, such as monocytes/macrophages and dendritic cells. IL-10 has important suppressive functions on immune responses and is believed to be involved in the maintenance of tolerance. IL-10 blocks activation of cytokine synthesis by TH1 cells, activated monocytes, and NK cells. It can stimulate the immunoglobulin production by B cells.
Downstream applications
IL-10 Secretion Assays were used to analyze IL-10-secreting, CMV-specific CD4+ T cells after stimulation with CMV lysate. The IL-10 Secretion Assay was also used to analyze IL-10 secretion by allergen-specific T cells.1,2 Furthermore, it was used for the detection and isolation of antigen-specific IL-10-secreting type I regulatory T cells from patients with an autoimmune skin disease. The isolated cells were cloned, expanded and further analyzed for their regulatory function.3 |
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| Figure 1 |
PBMCs of a CMV+ donor were stimulated for 16 hours with CMV lysate. The responding cells were stained and isolated according to secretion of IL-10 using the IL-10 Secretion Assay - Cell Enrichment and Detection Kit.
In the stimulated sample, 329 IL-10-secreting CD4+ cells were enriched per 106 CD4+ T cells by using MS Columns and a MiniMACS™ Separator. In the unstimulated control sample, two IL-10-secreting CD4+ T cells were detected per 106 CD4+ T cells after enrichment. |
| Stimulated sample |
| A: Before enrichment |
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| B: After enrichment |
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| Unstimulated control |
| C: Before enrichment |
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| D: After enrichment |
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* Percentage represents frequency among CD4+ T cells.
** Percentage represents frequency among enriched cells. |
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| Figure 2 |
| PBMCs of a CMV+ donor were stimulated with CMV-lysate. The responding cells were stained for coexpression of cytokines using the IL-10 Secretion Assay - Detection Kit (PE) and IL-2 Secretion Assay - Detection Kit (APC). The plots show IL-10 and IL-2 secretion by viable CD4+ T cells. |
| A: Stimulated sample |
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| B: Unstimulated control |
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| * Percentage represents frequency among CD4+ T cells. |
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| Details |
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| Products |
| IL-10 Secretion Assay - Cell Enrichment and Detection Kit (PE), human |
- for 50 tests with 107 total cells
Download datasheet
130-090-435
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| References |
| 1. Akdis et al. (2004) J. Exp. Med. 199: 1567-1575. |
| 2. Reefer et al. (2004) J. Immunol. 172: 2763-2772. |
| 3. Veldman et al. (2004) J. Immunol. 172: 6468-6475. |
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