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| IL-5 Secretion Assay – Cell Enrichment and Detection Kit |
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| Description |
| The IL-5 Secretion Assays - Cell Enrichment and Detection Kit was developed for the sensitive detection as well as the enrichment of human IL-5-secreting cells. |
| Details |
Background information
Interleukin-5 (IL-5) is predominantly produced by CD4+ T cells. IL-5 secretion has also been reported for γ/δ T cells, mast cells, NK cells, NKT cells, and eosinophils.
The cytokine was originally identified by its activity as a B cell growth factor and IgA-enhancing factor. Furthermore, IL-5 regulates growth, differentiation, activation, and survival of eosinophils from bone marrow into peripheral circulation (e.g. into lung following allergen exposure). It is involved in a range of allergic reactions and mediates immune reactions against parasites. |
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| Figure 1 |
| Human PBMCs were stimulated for 1 hour with CytoStim or left untreated. The responding cells were stained and isolated according to secretion of IL-5 using the IL-5 Secretion Assay - Cell Enrichment and Detection Kit. In the stimulated sample, 266 IL-5-secreting CD4+ cells were enriched per 106 CD4+ T cells by using MS Columns and a MiniMACS™ Separator. In the unstimulated control sample, one IL-5-secreting CD4+ T cell was detected per 106 CD4+ T cells after enrichment. |
| Stimulated sample |
| A: Before enrichment |
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| B: After enrichment |
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| Unstimulated control |
| C: Before enrichment |
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| D: After enrichment |
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| * Percentage represents frequency among CD4+ T cells. |
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| Details |
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| Products |
| IL-5 Secretion Assay – Cell Enrichment and Detection Kit (PE), human |
- for 50 tests with 107 total cells
Download datasheet
130-091-622
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