IL-17 Secretion Assay – Cell Enrichment and Detection Kit
Description Interleukin 17 (IL-17) is produced by CD4+ T helper cells, a T cell subset termed Th17, which also secrete IL-17F and IL-22 and express the NK cell marker CD1611. IL-17 secretion has also been described for other cell types, such as CD8+ memory T cells. Th17 cells are involved in the recruitment of neutrophils to control early stages of infections by a number of pathogens, such as extracellular bacteria and fungi. IL-17 and Th17 cells play an important role in immune-mediated inflammatory diseases, such as rheumatoid arthritis, psoriasis, multiple sclerosis, asthma, and inflammatory bowel disease. The IL-17 Secretion Assay – Cell Enrichment and Detection Kit allows the detection and isolation of viable human IL-17–secreting leukocytes from PBMCs. Applications Detection and enrichment of viable IL-17–secreting leukocytes from whole blood, PBMCs, or other leukocyte-containing single-cell preparations. Columns For positive selection using the IL-17 Secretion Assay – Cell Enrichment and Detection Kit: MS, LS or autoMACS® Columns.		PBMCs were stimulated with CytoStim for 4 hours or left untreated. Both samples were treated with a CD40 antibody to allow the surface detection of CD154. Cells were enriched by using the IL-17 Secretion Assay – Cell Enrichment and Detection Kit. The dot plots show stimulated and unstimulated cells before and after separation. Secretion of IL-17 by CD154+ cells is demonstrated. Data were gated on viable CD4+ cells. Restimulated sample A: Before enrichment B: After enrichment Without restimulation A: Before enrichment B: After enrichment

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