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| IL-17 Secretion Assay – Cell Enrichment and Detection Kit |
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| Description |
| The IL-17 Secretion Assay – Cell Enrichment and Detection Kit was developed for the sensitive detection and enrichment of viable human IL-17–secreting leukocytes from PBMCs. |
| Details |
Background information
Interleukin 17 (IL-17) is produced by CD4+ T helper cells, a T cell subset termed Th17, which also secrete IL-17F and IL-22 and express the NK cell marker CD1611. IL-17 secretion has also been described for other cell types, such as CD8+ memory T cells.
Th17 cells are involved in the recruitment of neutrophils to control early stages of infections by a number of pathogens, such as extracellular bacteria and fungi. IL-17 and Th17 cells play an important role in immune-mediated inflammatory diseases, such as rheumatoid arthritis, psoriasis, multiple sclerosis, asthma, and inflammatory bowel disease. |
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| PBMCs were stimulated with CytoStim for 4 hours or left untreated. Both samples were treated with a CD40 antibody to allow the surface detection of CD154. Cells were enriched by using the IL-17 Secretion Assay – Cell Enrichment and Detection Kit. The dot plots show stimulated and unstimulated cells before and after separation. Secretion of IL-17 by CD154+ cells is demonstrated. Data were gated on viable CD4+ cells. |
| Restimulated sample |
| A: Before enrichment |
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| B: After enrichment |
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| Without restimulation |
| A: Before enrichment |
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| B: After enrichment |
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| Details |
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| Products |
| IL-17 Secretion Assay – Cell Enrichment and Detection Kit (PE), human |
- for 50 tests with 107 total cells
Download datasheet
130-094-542
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| References |
| 1. Cosmi et al. (2008) J. Exp. Med. 205: 1903-1916. |
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