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| MC CD19 B Cell Cocktail (human) |
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| Description |
| The MC Antibody Cocktails further simplify the flow cytometric evaluation of cells separated by MACS® Technology. The MC CD19 B Cell Cocktail is designed to enable easy and rapid evaluation of MACS Separations using either CD19 MicroBeads, human, CD20 MicroBeads, human, or the B Cell Isolation Kit II, human. The antibodies are optimized for separation control using the MACSQuant™ Analyzer, enabling automated gating and analysis. Manual gating on other flow cytometers is also easily possible. MC CD19 B Cell Cocktail consists of CD19 and CD20 antibodies for reliable identification of B cells. In addition, a VioBlue-conjugated CD45 antibody is included as a trigger to restrict analysis to leukocytes. VioBlue requires the use of a violet laser. |
| Applications |
| The MC CD19 B Cell Cocktail is designed for separation control after cell separation using MACS® CD19 MicroBeads, human, CD20 MicroBeads, human, CD22 MicroBeads, human, or the B Cell Isolation Kit II, human. |
| Clone | Isotype |
| CD19-PE | Mouse IgG1 |
| CD20-APC | Mouse IgG1 |
| CD45-VioBlue | Mouse IgG2a |
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| Figure 1 |
| Human B cells were isolated from PBMCs using CD19 MicroBeads. Aliquots of cells before and after the separation were stained with the MC CD19 B Cell Cocktail and analyzed by flow cytometry. Dead cells and cell debris were excluded from the analysis by PI staining and scatter signals. |
| Before separation |
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| After separation |
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| Products |
| MC CD19 B Cell Cocktail, human |
- For 50 tests (1)
Download data sheet
130-092-860
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| (1) One test corresponds to fluorescent labeling of up to 107 cells in a total volume of 100 µL. |
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