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| MC CD14 Monocyte Cocktail (human) |
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| Description |
| The MC Antibody Cocktails further simplify the flow cytometric evaluation of cells separated by MACS® Technology. The MC CD14 Monocyte Cocktail is designed to enable easy and rapid evaluation of MACS Separations using either CD14 MicroBeads, human or the Monocyte Isolation Kit II, human. The antibody cocktail is optimized for separation control using the MACSQuant™ Analyzer, enabling automated gating and analysis. Manual gating on other flow cytometers is also easily possible. MC CD14 Monocyte cocktail consists of CD14 and CD15 antibodies for identification of monocytes and exclusion of unwanted cells. In addition, a VioBlue-conjugated CD45 antibody is included as a trigger to restrict analysis to leukocytes. VioBlue requires the use of a violet laser. |
| Applications |
| The MC CD14 Monocyte Cocktail is designed for separation control after cell separation using MACS® CD14 MicroBeads, human, or the Monocyte Isolation Kit II, human. |
| Clone | Isotype |
| CD14-PE | Mouse IgG2a |
| CD15-APC | Mouse IgM |
| CD45-VioBlue | Mouse IgG2a |
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| Figure 1 |
| Human monocytes were isolated from PBMCs using CD14 MicroBeads. Aliquots of cells before and after the separation were stained with the MC CD14 Monocyte Cocktail and analyzed by flow cytometry. Dead cells and cell debris were excluded from the analysis by PI staining and scatter signals. |
| After separation |
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| Before separation |
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| Products |
| MC CD14 Monocyte Cocktail, human |
- For 50 tests (1)
Download data sheet
130-092-859
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| (1) One test corresponds to fluorescent labeling of up to 107 cells in a total volume of 100µL. |
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