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| Blood Dendritic Cell Enumeration Kit (human) |
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| Description |
The Blood Dendritic Cell Enumeration Kit was developed for easy identification and counting of dendritic cells and dendritic cell subsets in whole blood or PBMCs by flow cytometry. It is based on the dendritic cell-specific surface antigens CD303 (BDCA-2), CD141 (BDCA-3), and CD1c (BDCA-1).1 The markers allow easy identification of three distinct dendritic cell subsets in blood: plasmacytoid dendritic cells (PDCs) are identified by CD303 (BDCA-2), type-1 myeloid dendritic cells (MDC1s) are identified by CD1c (BDCA-1), and type-2 myeloid dendritic cells (MDC2s) are identified by CD141 (BDCA-3) expression. The distinct dendritic cell subsets are analyzed within one sample by three-color (PDCs and MDC1s) or four-color (PDCs, MDC1s and MDC2s) flow cytometry.
The kit includes all reagents needed for a complete analysis: pre-mixed antibody cocktails for dendritic cell detection and for isotype control, a dead cell discrimination dye, and ready-to-use reagents for erythrocyte lysis and cell fixation. The standardized reagents and protocol ensure reproducible results.2 The dead cell dye used in this kit allows staining of dead cells in combination with cell fixation. This is particularly useful when working with hazardous samples.
The assay requires only small samples (either 300 µL of whole blood or 1×106 PBMCs, both for sample and control). |
| Applications |
| Identification and enumeration of peripheral blood PDCs and/or MDCs for monitoring of dendritic cell frequency and number, for example, after hematopoietic stem cell mobilization3, or in the course of pathogenic infections, for example by HIV.4 Furthermore, dendritic cell numbers were determined in patients with chronic graft versus host disease5, inflammatory bowel disease6, psoriatic arthritis and rheumatoid arthritis10, under long-term immunosuppression8, lymphoproliferative disease of granular lymphocytes9, or in patients with hepatocellular carcinoma, in order to analyze the immunosuppressive role of IL-10 on circulating dendritic cells7. |
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| Figure 1 |
| Dendritic cell subset–specific antigens. |
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| Figure 2 |
| Staining procedure of the Blood Dendritic Cell Enumeration Kit. |
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| Products |
| Blood Dendritic Cell Enumeration Kit, human |
for 50 tests (1)
Download data sheet
130-091-086
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for 2Ă—50 tests (1)
Download data sheet
130-091-324
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| (1) One test corresponds to fluorescent labeling of 2Ă—300 ÎĽL whole blood or 2Ă—106 PBMCs. |
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| MACS References |
| 1. Dzionek et al. (2000) J. Immunol. 165: 6037–6046.[898] |
| 2. Dzionek et al. (2002) Human Immunol. 36: 1133–1148.[2423] |
| 3. Arpinati et al. (2002) Bone Marrow Transplant. 29: 887–891.[2237] |
| 4. Chehimi et al. (2002) J. Immunol. 168: 4769–4801.[2301] |
| 5. Clark et al. (2003) Transplant. 75: 221–225.[2873] |
| 6. Baumgart et al. (2005) Gut 54: 228–236.[6527] |
| 7. Beckebaum et al. (2004) Clin. Cancer Res. 10: 7260–7269.[6539] |
| 8. Hackstein et al. (2005) J.Transplant. 5: 2945–2953.[8585] |
| 9. Zambello et al. (2005) Blood 106: 3926–3931.[8584] |
| 10. Jongbloed et al. (2006) Arthritis Research & Therapy 8: R15[9576] |
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