Treg Detection Kits (human)
Description The Treg Detection Kits, human, have been developed for the convenient detection of CD4+CD25+FoxP3+ regulatory T (Treg) cells by flow cytometry. Treg cells are a subset of T cells with the ability to suppress harmful immunological reactions to self and foreign antigens. Due to the lack of a specific marker for Treg cells, a combination of CD4, CD25, and FoxP3 is commonly used for their identification. The Treg Detection Kit includes all reagents necessary for cell surface staining of CD4 and CD25, and intracellular staining of FoxP3 with a special FoxP3 Staining Buffer Set. The kit also includes FcR Blocking Reagent and an optimized protocol. Applications Flow cytometric identification and enumeration of human CD4+CD25+FoxP3+ cells. Clone Isotype Anti-FoxP3 Mouse IgG1 CD4 (VIT4) Mouse IgG2a CD25 Mouse IgG2b		Figure 1 Human PBMCs were stained with the Treg Detection Kit (APC) and analyzed by flow cytometry. For the dot plot that shows CD25-PE and Anti-FoxP3-APC staining, gating was performed according to CD4-FITC staining. Figure 2 Human PBMCs were stained with the Treg Detection Kit (PE) and analyzed by flow cytometry. For the dot plot that shows CD25-APC and Anti-FoxP3-PE staining, gating was performed according to CD4-FITC staining.

Search this website

Advanced search